摘要
本文对南昌霉素 A 效价的生物和化学的测定方法分别进行了研究.生物测定方面筛选到对南昌霉素 A 的敏感菌、最适培养基及 pH 缓冲液.在5～50微克/毫升浓度范围内,敏感菌的抑菌圈直径与南昌霉素 A 的浓度的对数呈线性关系.化学测定方面探索到使南昌霉素 A 乙醇溶液显色的显色剂,摸清了最适显色条件和待测样品的处理方法.南昌霉素 A 与显色剂作用后的溶液显红色,颜色稳定,特异性强,显色后的溶液在721分光光度计上的最大吸收波长为520毫微米,在5～80微克/毫升浓度范围内其吸光度与南昌霉素 A 的浓度成正比,符合朗伯(Lambert)-比耳(Beer)定律。采用南昌霉素 A 标准品加入法和标准曲线法两种方法分别测定同一发酵液中南昌霉素 A 的含量,所获得的测定结果非常接近,表明本方法的置信度达到99%,样品效价测定的全过程可在1小时内完成.本研究结果为南昌霉素 A 发酵研究提供了较为简单、快速、准确的测定方法.
This paper reports a preliminary study on the microbiological and che-mical assay methods for the determination of nanchangmycin A.A sensitivebacterial strain,a more effective medium and a better buffer for pH valuehave been screened out.The diameter of inhibition zone of the sensitivestrain shows in Iinear correlation with the logarithm of the concentrationof nanchangmycin A in solution when it lies in the range of 5～50μg/ml.Onthe aspect of chemical assay,an agent which can make the ethanol solutionof nanchangmycin A become coloured has been found.The best procedurefor sample treatment and the optimum conditions for colour appearance havebeen determined.Nanchangmycin A solution becomes red after reacting withthis agent.This reaction is stable and highly specific.The wave length ofabsorption in 721 spectrophotometer is 520μm.As the concentration of na-nchangmycin A lies in the range from 5～80 μg/ml the absorbence value is indirect proportion to the concentration of nanchangmycin A,correspondentwith Lambert and Beer's law.The results obtained by using standard sam-ple adding method and standard curve method are almost identical,indicat-ing that the possibility of confidence reaches 99%.The whole precedure canbe completed within one hour.This study provids a simple,high-speed andaccurate assay method for the determination of nanchangmycin A.
出处
《江西农业大学学报》
CAS
CSCD
1990年第4期15-22,共8页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金
