摘要
目的测定清炒白术中白术内酯的含量。方法采用外标法，Dikma Kromasil 100AC18（4．6mm×250mm，5μm）色谱柱，乙腈-水梯度洗脱（60％～100％乙腈），流速为1．0mL·min，柱温25℃，检测波长为220nm（Ⅰ、Ⅲ）、276nm（Ⅱ）。结果测得4批清炒白术中白术内酯Ⅰ、Ⅱ、Ⅲ含量合计分别是：1．3261、1．5910、1．5348和1．7380mg·g^-1结论白术清炒后对所含白术内酯Ⅰ、Ⅱ、Ⅲ的含量有一定影响。
Objective To determine atractylenolides Ⅰ, Ⅱ, andⅢ in simple parching Rhizorrva Atractylodis macrocephalae by HPLC. Methods An external method was used with Kromasil C18 （4.6 mm×250 mm, 5μm） column and the mobile phase was composed of acetonitrile-water with a linear gradient elution. The flow rate was 1.0 mL·min^-1 with DAD detected at 220 nm for atractylenolides Ⅰ, Ⅲand 276 nm for atractylenolide Ⅱ. The column temperature was maintained at 25℃. Results Atractylenolides Ⅰ, Ⅱ, andⅢ in 4 batches simple parching on Rhizoma Atractylodis macrocephalae was 1. 326 1, 1. 591 0, 1. 534 8, and 1. 738 0 mg·g^-1. Conclusion Simple parching and ajuvants show some influence on the content of Rhizoma Atractylodis macrocephalae.
出处
《中南药学》
CAS
2009年第5期321-323,共3页
Central South Pharmacy
基金
广东食品药品职业学院2008年度项目(项目编号:2008Z009)
关键词
清炒白术
白术内酯Ⅰ白术内酯Ⅱ
白术内酯Ⅲ
高效液相色谱法
simple parching Rhizorru2 Atractylodls macrocephalae
atraetylenolides Ⅰ
atractylenolides Ⅱ
atractylenolilesⅢ
HPLC
