摘要
目的制备表皮生长因子(EGF)微球并对其生物学活性进行评价。方法利用改进的复乳法,以聚乳酸/羟基乙酸共聚物(PLGA)作为载体,制备EGF微球。检测EGF微球形貌表征、微球粒径分布、载药率、包封率和释药行为。用噻唑蓝(MTT)法测定增殖度,研究不同浓度EGF微球对细胞增殖能力的影响,研究相同浓度不同剂型EGF对细胞增殖的影响,研究微球载体的安全性。结果EGF微球粒径约为200nm,粒径分布比较均一,微球之间无粘连,分散性好。载药率为0.02%,包封率为85%。释药符合释放动力学模型,释放长达24h。不同浓度EGF微球均促进细胞增殖,其中10μg/L为最适质量浓度(与对照组比,P<0.01)。质量浓度10μg/L时,EGF微球组与EGF原液组相比差异有统计学意义(P<0.01)。不同质量浓度空微球对细胞没有毒性,不影响细胞增殖(组间没有差异,P>0.05)。结论成功制备EGF微球。细胞实验证明EGF微球制备过程中EGF保持原有活性,与EGF原液比较,EGF微球促进细胞增殖能力更强,微球载体对细胞没有毒性作用。
Objective To prepare epidermal growth factor（EGF） microsphere and evaluate its shape,particle diameter,drugloading efficiency, encapsulation ratio, release and biologic activity. Methods The modified multiple emulsion technique with polylactic-co-glycolic acid（PLGA） as carrier for preparing EGF microsphere was applied. The exosyndrome of EGF microsphere, diameter distribution,drug-loading efficiency, encapsulation ratio and release were determinened. The influence of cell proliferation with different concentration EGF microspheres was studied. The influence of cell proliferation with same concentration but different dosage form EGF was observed. The toxicity of particulate carrier for cell and influence on cell proliferation was researched. Results It was showed that the particle diameter of EGF microsphere was about 200 nm, particle diameter distribution was relative uniform, no conglutination among EGF microspheres, and dispersibility was good. The drugloading efficiency was 0.02 % ;encapsulation ratio was 85 %. The drug release behavior was coincided with Higuchi release kinetic model and lasted 24 hours. All of different concentration EGF microspheres could promote cell proliferation, but 10 μg/L was the optimal concentration （vs control group ,P 〈 0.01）. The effects of different dosage form EGF with same concentration of 10 μg/L had difference. The EGF microsphere group was better than EGF stock solution group（P 〈 0.01）. The different concentration empty microspheres showed no effect on cell proliferation （P 〉 0.05） and toxicity was not found. Conclusion The EGF microsphere is successfully prepared. It is demonstrated in cell experiment that EGF keeps good activity in EGF microsphere. The effect of EGF microsphere on promoting cell proliferation capacity is better than that of EGF stock solution. Toxic effect of particulate carrier on cells is not found.
出处
《生物医学工程与临床》
CAS
2009年第3期175-179,共5页
Biomedical Engineering and Clinical Medicine
关键词
表皮生长因子
微球制备
生物学活性
epidermal growth factor
microsphere preparation
biologic activity
