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胆色素原脱氨酶的研究进展 被引量：1

Development in the Research on Porphobilinogen Deaminase
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摘要 胆色素原脱氨酶是生物体内参与四吡咯化合物生物合成过程的关键酶之一,它催化了4个底物脱去4分子氨依次连接成线形的四吡咯产物羟甲基胆素.论文总结了胆色素原脱氨酶在生化性质、晶体结构、催化机理等方面的研究成果,并介绍了该酶的一些重要氨基酸. Porphobilinogen deaminase （PBGD） is one of the key enzymes which participate in the biosynthesis of the tetrapyrroles. PBGD catalyzes the formation of the linear tetrapyrrole hydroxymethylbilane from four molecules of monopyrrol substrate, with the elimination of four molecules of NH3. The results of research on the biochemical nature, crystal structure and catalytic mechanism are summarized in this article.
作者 李楠
机构地区 五邑大学化学与环境工程系
出处 《五邑大学学报（自然科学版）》 CAS 2009年第2期15-19,共5页 Journal of Wuyi University(Natural Science Edition)
基金 广东高校优秀青年创新人才培育项目资助(LYM08096)
关键词 胆色素原脱氨酶 四吡咯 晶体结构 催化机理 porphobilinogen deaminase tetrapyrroles crystal structure catalytic mechanism
分类号 Q557.3 [生物学—生物化学]
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参考文献21

  • 1BATTERSBY A R, LEEPER F J. Biosynthesis of the pigments of life-mechanistic studies on the conversion of porphobilinogen to uroporphyrinogen-Ⅲ[J]. Chemical Reviews, 1990, 90: 1 261-1 274.
  • 2SHOOLINGIN-Jordan P M. Porphobilinogen deaminase and uroporphyrinogen Ⅲ synthase: structure, molecular biology, and mechanism[J]. Journal of Bioenergetics and Biomembranes, 1995, 27: 181-195.
  • 3ANDERSON P M, DESNICK R J. Purification and properties of uroporphyrinogen 1 synthase from human erythrocytes[J]. Journal of Biological Chemistry, 1980, 255:1 993-1 999.
  • 4WILLIAMS D C, MORGAN G S, MCDONALD E, et al. Purification of porphobilinogen deaminase from Euglena gracilis and studies of its kinetics[J]. The Biochemical Journal, 1981, 193:301-310.
  • 5HART G J, ABELL C, BATTERSBY A R. Purification, N-terminal amino acid sequence and properties of hydroxymethylbilane synthase (porphobilinogen deaminase) from Escherichia coli[J]. The Biochemical Journal, 1986, 240: 273-276.
  • 6NORIEGA G N, MATTEI G, BATLLE A, et al. Rat kidney porphobilinogen deaminase kinetics detection of enzyme-substrate complexes[J]. The International Journal of Biochemistry & Cell biology, 2002, 34: 1 230-1 240.
  • 7FUJINO E, FUJINO T, KARITA S, ct al. Purification and characterization of the Clostridium josui porphobilinogen dcaminas encoded by the hemC gene from a recombinant Escherichia coli[J]. Journal of Bioscience and Bioengineering, 1999, 87: 535-537.
  • 8GRANDCHAMP B, VERNRUIL H D, BEAUMONT C, et al. Tissue-specific expression of porphobilinogen deaminase[J]. European Journal of Biochemistry, 1987, 162: 105-110.
  • 9CARDALDA C A, BATLLE A, JUKNAT A A. Sequence and structure of the rat housekeeping PBGD isoform[J]. Biochemical and Biophysical Research Communications, 1998, 249: 438-443.
  • 10LI N, CHU X S, WU L, et al. Functional studies of rat hydroxymethylbilane synthase[J]. Bioorganic Chemistry, 2008, 36: 241-251.

同被引文献28

  • 1程冬梅,范三红,刘香莉,陈根云,邓志勇,郭蔼光.小麦返白系胆色素原脱氨酶纯化及编码cDNA序列分析[J].中国生物化学与分子生物学报,2006,22(12):973-978. 被引量：3
  • 2Lin Y L,Lai Z X. Reference gene selection for qPCRanalysis during somatic embryogenesis in longan tree[J].Plant Science,2010,178(4):359-365.
  • 3Romanowski T ,Markiewicz A, Bednarz N, et al.Housekeeping genes as a reference in quantitativereal-time RT-PCR[J]. Postepy Hig Med Dosw,2007,61:500-510.
  • 4Vandesompele J,De Preter K, Pattyn F,et al. Accuratenormalization of real-time quantitative RT-PCR data bygeometric averaging of multiple internal control genes[J].Genome Biol,2002,3(7):RESEARCH0034.
  • 5Andersen C L,Jensen J L,Orntoft T F. Normalization ofreal-time quantitative reverse transcription-PCR data:amodel-based variance estimation approach to identifygenes suited for normalization,applied to bladder andcolon cancer data sets[J]. Cancer Res,2004,64(15):5245-5250.
  • 6Zhao X,Wang Q,Jiao Y,et al. Identification of GenesPotentially Related to Biomineralization and Immunity byTranscriptome Analysis of Pearl Sac in Pearl OysterPinctada martensii[J]. Mar Biotechnol,2012,14(6):730-739.
  • 7Michael W. Pfaffl , Ales T , Christian P , et al.Determination of stable housekeeping genes ,differentially regulated target genes and sampleintegrity:BestKeeper-Excel-based tool using pair-wisecorrelations[J]. Biotechnol Lett,2004,26(6):509-515.
  • 8Nicholas S , Steve B , Jie J , et al. Selection ofhousekeeping genes for gene expression studies inhuman reticulocytes using real-time PCR[J]. BMCMolecular Biology,2006,7:33.
  • 9Borowski J,Galli V,Messias R S,et al. Selection ofcandidate reference genes for real-time PCR studies inlettuce under abiotic stresses[M]. Planta,2014,239(6):1187-1200.
  • 10Lossos I,Czerwinski D,Wechser M,et al. Optimizationof quantitative real-time RT-PCR parameters for thestudy of lymphoid malignancies[J]. LEUKEMIA,2003,17(4):789-795.

引证文献1

五邑大学学报（自然科学版）
2009年 第2期

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