摘要
目的探讨Wnt通路抑制因子Dickkopf-1(DKK1)在人胶质瘤发生、发展中的作用。方法采用ELISA、RT-PCR法检测人胶质瘤细胞株U251和T98G中DKK1蛋白及DKK1 mRNA表达水平。结果DKK1蛋白在U251和T98G中均呈高表达;DKK1mRNA在胶质瘤细胞株U251和T98G中高表达,但在正常脑组织中表达不明显。结论DKK1在人胶质瘤细胞株中高表达;其可能在胶质瘤发生发展中起重要作用。
Objective To investigate the significance of dickkopf1 （ DKK1 ） expression in human glioma cell lines U251 and T98G. Methods The expression of DKK1 protein and DKK1 mRNA in human glioma cell lines U251 and T98G were detected by enzyme-linked immunosorbant assay （ELISA） and reverse transeriptase-polymerase chain reaction （RT- PCR）. Results There were high expression of DKK1 protein and DKK1 Mma in both U251 and T98G,but not in normol brain tissues. Conclusion DKK1 is over-expressed in glioma cell lines which may play an important role in the initiation and development of glioma.
出处
《山东医药》
CAS
北大核心
2009年第19期23-24,共2页
Shandong Medical Journal
基金
江苏省卫生厅重大项目(K200508)。
