摘要
取江西省儿童医院132名腹泻住院儿童粪便,经处理和抗酸染色后,发现17份有类似隐孢子虫卵囊.后取17份中的2价标本,经人工感染小鼠,未能证明其致病性.后又分别将17份可疑阳性的粪便样品接种于沙堡弱琼脂平板上,室温培养24h,结果以感染小鼠粪便及沙堡弱琼脂上均分离出酵母菌.培养出的酵母菌经抗酸染色,发现少数酵母菌也可呈现阳性,故以抗酸染色法检查粪便中的隐孢子虫卵囊时要注意与酵母菌相区别.这次132例中未能证实其中有隐孢子虫.
One hundred and thirty two diarrheal samples of hospitalized infants in Jiangxi Provincial Children's Hospital were collected. After a treatment by saturated NaCl solution and stained by acid-fast staining, 17 samples were suspected of cryptosporidium oocyte. Two of the 17 samples were inoculated into mice and sabouraud medium. From sabouraud medium it was found that there was a rich growth of a typical yeast. Most of the yeast were nonacid-fast, but a few were acid-fast. All the mice were healthy. The same yeast was recovered from feces of inoculated mice by cultivation on sabouraud medium while feces of uninoculated mice control did not. Therefore, we did not prove the presence of any cryptosporidium oocyte among those samples. Laboratory workers should pay attention to differentiate the yeast from the cryptosporidium oocyte when fecal samples stained with acid-fast staining.
出处
《江西科学》
1990年第3期55-58,共4页
Jiangxi Science
关键词
孢子虫
卵囊
腹泻
儿童
粪便
Cryptosporidium, Cryptosporidium oocyi:e, Diarrheal children,Feces
