摘要
通过室内培养,研究了不同亚硝态氮浓度对铜绿微囊藻(Microcystis aeruginosa)生长的影响和藻对亚硝态氮的利用,实验分析了水体中亚硝态氮、硝态氮和铵态氮浓度的变化,测定了铜绿微囊藻的生长曲线、藻细胞内亚硝态氮含量和藻亚硝酸氧化酶(NOR)。结果显示,在10mgNO2--N·L-1的处理组中,培养基中亚硝态氮和硝态氮浓度同时减少,说明铜绿微囊藻可以同时利用亚硝态氮和硝态氮;在20和30mgNO2--N·L-1的处理组中,随着藻的生长培养基中亚硝态氮的浓度减少,硝态氮浓度增加,而且电泳实验显示此培养条件下铜绿微囊藻能产生亚硝酸氧化酶,表明培养基中的亚硝态氮被亚硝酸氧化酶氧化为硝态氮。本实验也表明高浓度的亚硝态氮(大于10mgNO2--N·L-1)能够抑制藻的生长。
To investigate the effect of nitrite on growth of Mieroeystis aeruginosa and utilization of nitrite by cyanobacteria, the growth curve, the intracellular nitrite content and nitrite oxidoreductase （NOR）of M. aeruginosa under different nitrite eoneentration and the changes of con- centrations of nitrite, nitrate and ammonium in the culture medium had been measured. Our results showed that both nitrite concentration and nitrate concentration decreased with cyanobaeteria culturing in the medium containing 10 mg NO^-2-N·L^-1, meanwhile, intracellular iaitrite concentration increased with increase of nitrite concentration in euhure medium, indicated that M. aeruginosa could uptake nitrite and nitrate simultaneously, moreover, the increase of nitrite concentration could promote nitrite uptake. However, in medium of 20, 30 mg NO^-2-N·L^-1, the nitrate concentration increased with nitrite concentration decreasing, and NOR was observed, revealing the nitrite might he oxidated to nitrate by NOR. Our results also showed that the growth of cyanobacteria was inhibited under high nitrite concentrations（over 10mg NO^-2-N·L^-1）.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第5期989-992,共4页
Journal of Agro-Environment Science
基金
天津大学-南开大学合作基金(AJ0013)
