摘要
从利凡诺去白蛋白人血浆中提纯了蛋白C抑制物，抗血清由免疫家兔产生，“’互一蛋白C抑制物用Iodogen法制备，采用平衡法建立RIA，数据用四参数logistic数据处理程序处理。方法的批内和批间CV分别为2.73％和8.62％，平均回收率为101.89％，可测范围为4．8～1024pg／L，ED50为19.34μg／L。40名健康男、女性血浆蛋白C抑制物含量为4．69土1．8mg／L（），本方法可为血栓、栓塞性疾病的诊疗和蛋白C系统的基础及临床研究提供一种有效的手段。
A radioimmunoassay of protein C inhibitor(PCI) was established. PCI was purified from albumin free human plasma by rivanol precipitation, and the antiserum was obtained byimmunizing the rabbits. The 125I - PCI was prepared by the lodogen method. The data wereprocessed by the four - parameter logistic RIA program. Methodological study found that the intra- and inter - C. V. was 2. 73 % and 8. 62 %, respectively, and the recovery rate was 101. 89 %.The range of measurement was 4. 8 ～ 1024μg／L, and ED5o was 19. 34 μg／L. No cross - reactivitywas found with thrombin, antithrombin Ⅲ， protein C, protein S and thrombomodulin. Humanplasma samples were assayed and the plasma PCI level of 40 healthy volunteers was 4. 69 ±1.sing／L(x ±2s ). This new method can be used as an effective tool especially for diagnosis ofthrombosis and the basic or clinical study of protein C system.
出处
《标记免疫分析与临床》
CAS
1998年第1期38-41,共4页
Labeled Immunoassays and Clinical Medicine
基金
国家自然科学基金
中国核工业总公司科研基金资助
