摘要
为了解新生儿肺炎IL－2R的变化，采用ELISA法检测33例新生儿肺炎血清sIL－2R水平、PBMC体外PHA培养72小时mIL－2Rα表达及上清液sIL－2R水平，并与36例正常新生儿对照。结果：（1）患儿血清sIL－2R水平治疗前高于治疗后（P＜0.001），且治疗前后均高于对照（P＜001）；治疗前血清sIL－2R水平与病情轻重密切相关（P＜0．01）；（2）患儿mIL－2Rα表达治疗前后无显著性差异（P＞005），上清液SIL－2R水平治疗前低于治疗后（P＜0．05）；而治疗前后两者均低于对照（P＜0.05）。提示：血清sIL－2R水平可作为新生儿肺炎病情监视及疗效观察的参考指标；患儿PBMC在体内可能已被激活，在体外对PHA非特异刺激的敏感性降低。
To investigate the change of neonatal immune function, 33 neonates with pneumonia had serum sIL - 2R levels determined by ELISA, and after their PBMCs were cultured invitro with PHA for 72 hours, the supernatant sIL - 2R and the extraction of mIL - 2Ra were alsomeasured by ELISA. 36 normal neonates were taken as control. Result: 1 ) The serum levels ofsIL - 2R in pre - treated patients were higher than those of post - treated patients, and levels ofboth pre - and post - treated were higher than those of controls; The serum levels of sIL - 2Rwere closely correlated with the seventy of the disease(γ = 0. 85, P＜ 0. 01 ); 2 )There was no significant difference in the mIL - 2Rα expression between the pre - and post - treated patients(P ＞0. 05). The supernatant sIL - 2R levels in pre - treated patients were lower than those of Post treated patients. The present findings suggest: serum sIL - 2R level might serve as a reference index for assessing the severity of disease and therapeutic response in neonatal pneumonia; PBMCsfrom patients were possibly activated in vivo so as to have a low respones to non - specific stimulation of PHA in vitro.
出处
《标记免疫分析与临床》
CAS
1998年第1期10-13,共4页
Labeled Immunoassays and Clinical Medicine
