摘要
研究了碳源、氮源、各种理化因素以及培养条件对白腐菌TP21漆酶分泌的影响。结果表明:木糖和多糖作碳源、酵母膏作氮源有利于漆酶的分泌,pH在4.0-6.0的范围内对漆酶的分泌影响不大,培养温度、接种量、通气量对漆酶的分泌有较大影响,培养基中添加诱导物对漆酶的产生有促进作用,特别是玉米秸秆价格低廉,是较好的选择。
The effect of carbon source/nitrogen source/physicochemical element ano culture cuuu on laccase production of white-rot fungus TP21 was studied. The results showed that starch and yeast paste were better to be used as carbon source and asnitrogen source respectively. There was little difference of effect of pH 4.0- 6.0 of the medium on laccase production, but the culture temperature, inoculation amount and ventilation volume had greater influenee on laecase production by the fungus. The curve of laccase production and optimal condition of laccase reaction were also described. Incubation temperature, inoculum concentration, minute ventilation volume have great effect on laccase production. Additive inducer in culture medium has the promoter action to laccase production. Especially those maize straws are inexpensive ingredients which be selected.
出处
《西北农业学报》
CAS
CSCD
北大核心
2009年第3期249-253,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
北京市属市管高等学校“人才强教项目”资助
关键词
白腐菌
漆酶
培养条件
White-rot fungus
Laccase
Culture conditions