摘要
目的:研究人牙周膜成纤维细胞(human periodontal ligament fibroblasts,HPDLF)对盐酸林可霉素的跨膜转运,为根管牙周局部及全身给药提供实验依据。方法:盐酸林可霉素溶液孵育HPDLF和MC3T3-E1细胞,超声破碎细胞后,HPLC测定不同时间点的胞内药物含量,考马斯亮蓝法测定细胞蛋白总量。结果:10μg/ml的盐酸林可霉素孵育HPDLF及MC3T3-E1细胞1min、5min、10min时均无法检测出胞内药物的存在,20μg/ml的药物作用5min、10min,同样无法检测。结论:HPLC可以用于盐酸林可霉素的测定。本实验条件下不能检测出HPDLF以及MC3T3-E1细胞对盐酸林可霉素的跨膜转运。
Objective： To investigate biological transport of lincomycin hydrochloride by human periodontal ligament fibroblasts （HPDLF） to verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal. Method： HPDLF and MC3T3-E1 cells were incubated in antibiotics solutions. The intracellular antibiotics contents were measured by HPLC and the cell total protein was measured by Bradford protein assay. Result： The intracellular antibiotics content of HPDLF and MC3T3-E1 cells couldn＇t be detected at 1 min,5 min, 10 min,incubated in 10 ug / ml and 20 ug/ml antibiotics solutions. Conclusion：HPLC is an accurate,sensitive method for measurement of lincomycin hydrochloride. With the limitations, lincomycin hydrochloride can＇t be detected of transport by HPDLF.
出处
《临床口腔医学杂志》
2009年第5期268-270,共3页
Journal of Clinical Stomatology
关键词
人牙周膜成纤维细胞
盐酸林可霉素
生物转运
高效液相色谱法
human periodontal ligament fibroblasts
lincomycin hydrochloride
biological transport： high performance liquid chromatography
