摘要
目的检测EGFR基因在新疆卡波氏肉瘤（Kaposi’s Sarcoma，KS）中的表达并分析其-9KS发生发展的关系。方法应用SYBRGreenI作为荧光染料，以GAPDH作内参照，建立检测EGFRmRNA表达的实时荧光PCR反应体系，对经病理活检证实的7例KS肿瘤组织和瘤旁正常组织中EGFRmRNA的表达水平进行检测。结果KS肿瘤组织中EGFR表达水平低于瘤旁正常组织（t=-3．865，P〈0．05），并且结果有统计学意义。KS肿瘤组织中EGFR的相对表达量为0．34±0．31，瘤旁组织中为1．24±0．78（P=0．008）。结论所建立的SYBRGreenI实时定量PCR方法可以成功地检测EGFR基因的表达量。KS肿瘤组织中EGFR表达水平低于瘤旁正常组织，差异有统计学意义。
Objective To detect the expression of EGFR gene in Kaposi＇s sarcoma in Xinjiang and study the correlation of EGFR mRNA with the progression of KS. Methods Using the Fluorescence indicator SYBR Green I and an inner control GAPDH gene, an amplifying condition was established for detecting the expression levels of EGFR mRNA in KS and normal skin tissues of 7 KS patients confirmed by pathological biopsy. Results The expression of EGFR mRNA in KS was lower than in normal control group （t=-3.865, P〈0.05）. There was a significant difference in the results. The relative levels of EGFR mRNA expression in KS and normal skin tissues were 0.34±0.31 and 1.24±0.78 respectively （P=-0.008）. Conclusions The established SYBR Green I quantitative real-time PCR method can successfully detect the expression of EGFR mRNA. The expression of EGFR mRNA in KS was lower than in normal control group. The primary results show that EGFR may be a protective factor in oncogenesis and development of KS.
出处
《世界感染杂志》
2009年第2期90-93,共4页
World Journal of Infection
基金
基金项目：国际科技合作项目（2005DFA30780）,教育部科学研究重大项目（206166）
