摘要
应用反向点杂交法（ＲＤＢ）的原理，针对ＨＰＶ６Ｂ，１１，１６，１８，３１，３３和３５设计了７条序列作为未标记的特异性寡核苷酸（ＳＳＯ）探针，分别固定在尼龙膜条上，形成７个点，再与经ＰＣＲ扩增的样品ＤＮＡ序列杂交，即可在一个膜条上分辨出这７型ＨＰＶ中的任一型．此法快速简便，特异性高，不存在假阳性；且因ＰＣＲ灵敏度高，亦不易出现假阴性．用ＰＣＲＲＤＢ法检测保存的宫颈癌组织石蜡包埋标本３２例，结果：ＨＰＶ１６阳性２２例（６８８％），ＨＰＶ１８阳性５例（１５６％），ＨＰＶ１６／１８双重感染２例（６３％），阴性仅３例（９３％）．
Reverse dot blot (RDB) technique was used. Seven sequencespecific oligonucleotide (SSO) probes directed against 7 types of HPV (HPV6B, 11, 16, 18, 31, 33 and 35) respectively are synthesized and fixed sequentially on a stripe of nylon membrane to form 7 spots. After hybridization with the PCR product of the sample DNA sequence, any one of the 7 types of HPV DNA were distinguished on only one stripe of nylon membrane. 38 cases of cervical carcinoma samples were detected by this PCRRDB technique. The results show: 29 cases (763%) of HPV16 positive; 8 cases (211%) of HPV18 positive; 2 cases (72%)of double infection; and 3 cases (80%) of HPV negative.The method is rapid and simple, with high specificity, no false positive results in general, and as the PCR technique is very sensitive, the method is not liable to show false negative results.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第1期75-78,共4页
Progress In Biochemistry and Biophysics
关键词
反向点杂交
聚合酶链反应
子宫颈癌
reverse dot blot, polmerase chain reaction (PCR), cervical carcinoma, condyloma acuminatum, human papillomavirus (HPV)
