摘要
水稻卵细胞与合子的分离韩红梅赵洁施华中杨弘远周嫦（武汉大学生命科学学院植物生殖生物学研究室武汉４３００７２）关键词水稻，卵细胞，合子，分离ＩＳＯＬＡＴＩＯＮＯＦＥＧＧＣＥＬＬＳＡＮＤＺＹＧＯＴＥＳＩＮＯＲＹＺＡＳＡＴＩＶＡＨＡＮＨｏｎｇＭｅｉＺ...
A method of nonenzymatic,manual microdissection was established to isolate egg cells and zygotes in Oryza sativa L.Generally 5 to 8 protoplasts of egg cells or zygotes could be isolated from 20 ovaries in 2 h.Fluorochromatic reaction proved that the isolated cells were viable and could survive in the isolation medium consisting of 0.5 mol/L mannitol and 5 mmol/L CaCl2 for at least 2 h.The egg cells and early zygotes usually turned to spherical shape during the course of isolation,whereas the late zygotes often maintained pearshaped.The rounded zygotes were proved to be true protoplasts by Calcoflour White fluorescent staining.Chlorotetracycine and fluphenanize fluorescence treatment demonstrated that the isolated zygotes were rich in membranebound calcium as well as calmodulin.
基金
国家自然科学基金
欧洲委员会国际合作项目
关键词
水稻
卵细胞
合子
分离
Oryza sativa, Egg cell, Zygote, Isolation
