摘要
对常规染色体的观察结果表明：原初的小冰麦异附加系ＴＡＩ１４为２ｎ＝４４，其中所有的染色体都是中部或近中部着丝点染色体。但在其后代中发现有一对染色体变成了端着丝点染色体。为判明变异的染色体是冰草还是小麦的染色体，用荧光原位杂交技术进行了检测，结果表明，ＴＡＩ１４中的所有小麦染色体都显示红色荧光，只有一对端着丝点染色体显示绿色荧光，说明变异的是冰草染色体，即：小冰麦异附加系ＴＡＩ１４原为二体异附加系，现变成了双端体异附加系。对变异发生的原因和双端体异附加系的用途进行了讨论。
The karyotype of the primary wheatwheatgrass disomic addition line TAI14 was 2n=44 in which all of the chromosomes were metacentric and submetacentric. However, in the progeny of the TAI14, a pair of telocentric chromosomes were observed. In order to clarify whether the telocentric chromosomes were of common wheat or of wheatgrass, the fluorescence in situ hybridization (FISH) technique was employed. It was revealed that the wheat chromosomes exhibited red fluorescence while the ditelocentric chromosomes green fluorescence. Therefore, the primary TAI14 was conversed from the disomic addition line into the ditelocentric addition line. The possible explanation for such a variation and the potential significance of the ditelocentric addition line were discussed briefly.
关键词
小麦
天蓝冰草
双端体异附加系
荧光原位杂交
Common wheat, Wheatgrass, Ditelocentric alien addition line, Fluorescence in situ hybridization
