摘要
目的：探讨多胺生物合成关键酶ＯＤＣ的不可逆抑制剂ＤＦＭＯ对人早幼粒白血病细胞ＨＬ６０生长及凋亡的影响。方法：细胞形态观察、流式细胞分析、ＤＮＡ凝胶电泳及免疫细胞化学等。结果：ＤＦＭＯ不仅能阻止ＨＬ６０细胞生长，而且能诱导ＨＬ６０细胞凋亡，同时引起凋亡抑制基因ｂｃｌ２表达下调。此外用外源性腐胺与ＤＦＭＯ同时处理ＨＬ６０细胞可阻止ＤＦＭＯ所引起的上述变化。结论：ＤＦＭＯ阻断ＨＬ６０细胞的多胺生物合成可在一定程度上使ＨＬ６０细胞恶性表型发生逆转，提示多胺生物合成抑制是治疗肿瘤一种值得进一步研究的途径。
Objective:To observe the effect of DFMO,an inhibitor of ODCa key enzyme of polyamine biosynthesis,on the growth and apoptosis of HL60 cells.Methods:Cell morphological assay,flow cytomeric analysis、DNA fragmentation and immunocytochemical staining were used.Results:DFMO not only arrested HL60 cells growth,but also induced their apoptosis which was associated with down regulation of bcl2 gene expression.These changes resulted from DFMO were prevented,however,when the cells were treated by DFMO along with exogenous putrescine.Conclusion:The control of polyamine biosynthesis in HL60 by DFMO could reverse its malignant phenotypes to a certain extent and it is a worth search approach for tumor therapy.
出处
《中日友好医院学报》
1998年第1期17-20,共4页
Journal of China-Japan Friendship Hospital
