摘要
运用ＲＮＡ分子杂交的方法，观察了热休克蛋白（ｈｅａｔｓｈｏｃｋｐｒｏｔｅｉｎ，ＨＳＰ）９０α、７０、２７在２２例白血病病人细胞、正常血细胞及Ｋ５６２红白血病细胞系中的表达。结果表明：１６例白血病病人中，血细胞呈现ＨＳＰ２７高水平表达，较正常血细胞显著增多１３例，其中急性淋巴细胞白血病（ＡＬＬ）４例，急性非淋巴细胞白血病（ＡＮＬＬ）６例，慢性粒细胞白血病急变期（慢粒急变）１例和骨髓增生异常综合征（ＭＤＳ）２例，ＡＬＬ（５例）与ＡＮＬＬ（７例）白血病细胞ＨＳＰ２７表达水平无明显改变。检测了７例白血病细胞ＨＳＰ７０的表达水平，除１例ＡＬＬ及１例ＭＤＳ明显升高外，其余５例（包括１例ＡＬＬ，３例ＡＮＬＬ和１例慢性急变）显著低于正常血细胞。１７例白血病病人细胞（包括ＡＮＬＬ９例、ＡＬＬ５例、慢粒急变２例和ＭＤＳ１例）ＨＳＰ９０α表达水平均升高，明显高于正常。结果提示；白血病细胞ＨＳＰ９０α表达水平的升高可能与白血病细胞活跃异常增殖有关，而ＨＳＰ２７基因的高表达可能不是某种急性白血病的特殊标志。
The expression of three heat shock proteins(HSPs)HSP90α, HSP70, HSP27 in cells obtained from 22 patients with leukemia, K562 erythroleukemia cell line, and normal blood cells were observed by means of RNA Dot Blot analysis. The results showed that the expression of the HSP27 gene was enhanced in 4 cases with Acute Lymphoid Leukemia (ALL), 7 cases with Acute Nonlymphoid Leukemia(ANLL) and 2 cases with myelodysplastic syndrome(MDS) as compared wiht the normal blood cells, yet there was no significant differences on the HSP27 expression between the ALL and ANLL cells. The expression of HSP27 in all the 5 ALL and ANLL patients was much lower than the normal cells, except 1 case with ALL and 1 case with MDS, which enhanced obviously. All the cases including 11 ANLL, 5 ALL and 1 MDS had higher HSP90α expression than the normal cells. The enhanced expression of HSP90α in leukemia cells may be associated with the active and indefinite proliferation of leukemia cells. Our results also suggest that the high expression of the HSP27 gene is not confined to a specific type of acute leukemia.
出处
《白血病》
CAS
1998年第1期27-30,共4页
