摘要
目的研究硒对实验性大鼠肝纤维化的防治作用．方法Ｗｉｓｔａｒ大鼠８０只，体重１８０ｇ～２２０ｇ，随机分为４组：①正常对照组；②病理模型组，用人血白蛋白诱导肝纤维化，第１步皮下注射致敏４５ｄ，随之静脉注射；③硒防治组，静脉注射４ｗｋ后，以硒溶液灌胃；④秋水仙碱治疗组，静脉注射人血白蛋白１２ｗｋ后，秋水仙碱灌胃．静脉注射结束后，治疗开始后６ｗｋ和１２ｗｋ采集肝组织标本，用光镜和电镜进行肝脏组织学检查．结果硒防治组肝脏纤维化的程度（Ⅰ级２只，Ⅱ级３只，Ⅲ级３只，Ⅳ级１只）明显低于病理模型组（Ⅱ级２只，Ⅲ级３只，Ⅳ级５只，Ⅴ级３只，Ｐ＜００１）硒防治组肝脏超微结构的变化也较轻微．结论硒对实验性大鼠肝纤维化有一定的预防和治疗作用．
IM To investigate the effects of selenium in prevention and treatment of experimental liver fibrosis in rats.METHODS Eighty Wistar rats, weighing 180g-220g, were randomly divided into 4 groups: a. normal control group; b. pathological model group: liver fibrosis was induced by normal human serum albumin, subcutanous sensitization injection was made for 45 days, and followed by intravenous boost. c. prevention and treatment group: selenium liquid was administered by gastric gavage after intravenous boosts for 4 weeks. d. Colchicine treatment group: colchicine was started at the end of intravenous injection of human albumin for 12 weeks. Liver samples were obtained at 6 and 12 weeks and after intravenous boosts and studied histologically by HE stain, Mallory′s stain and EM.RESULTS The fibrosis grades in prevention and treatment group were lower (Grade Ⅰ-Ⅳ was in 2,3,3 and 1 rats, respectively) than that (Grade Ⅱ-Ⅴ was in 2,3,5 and 3 rats, respectively) in pathological model group (P<001). Ultrastructural changes were slight in prevention and treatment group.CONCLUSION Selenium can reduce the serverity of liver fibrosis in rats.
关键词
硒/药理学
肝硬变
预防
病理学
elenium/pharmacology
liver cirrhosis, experimental/prevention & control
liver/pathology
