摘要
为了探讨抗癌基因ｐ５３、癌转移抑制基因ｎｍ２３的表达产物在胃癌及其对应的淋巴转移灶和正常组织中的表达，应用抗鼠ｐ５３，ｎｍ２３Ｈ１单克隆抗体，用亲和素生物素过氧化物酶复合物法测定了这两种蛋白在上述组织中的表达．结果，在胃癌原发灶中ｎｍ２３蛋白阳性表达率为４１％，ｐ５３蛋白阳性表达率为５０％；在对应淋巴转移灶中ｎｍ２３蛋白阳性表达率为１１％，而ｐ５３蛋白阳性表达率为６７％．非癌组织中ｎｍ２３蛋白表达全部为阴性，ｐ５３蛋白阳性表达率为２／６．ｎｍ２３蛋白在胃癌原发灶中的阳性表达率显著高于非癌组织中的阳性表达率，胃癌原发灶中的阳性表达率显著高于转移灶中的ｎｍ２３蛋白阳性表达率，原发灶高分化胃癌中的ｎｍ２３蛋白阳性表达率显著高于低分化胃癌中的ｎｍ２３蛋白阳性表达率；ｐ５３蛋白在胃癌淋巴转移灶中的阳性表达率显著高于非癌组织中的阳性表达率．
We have studied the expression of antimetastatic gene nm23 product and antioncogene p53 protein in gastric carcinoma and its corresponding lymph nodes and nontumor tissues. By ABC method with nm23H1 and p53 monoclonal antibodies.the results are as follows: the positive expression rates of nm23 in the primary focal of gastric carcinoma,metastatis lymph nodes and the nontumor tissues are 41%, 11% and 0% respectively. The positive expression rates of p53 in the primary focal of gastric carcinoma, metastatis lymph nodes and the nontumor tissues are 50%,67% and 2/6 respectively.The expression rates of nm23 protein in the gastric carcinoma are higher than those in nontumor tissues and me tastatis lymph nodes, and higher in well differentiation gastric carcinoma tissues than in poorly ones.The expression rate of p53 protein is higher in the metastatis lymph nodes than that in nontumor tissues.
出处
《延边大学医学学报》
CAS
1998年第1期1-4,共4页
Journal of Medical Science Yanbian University
关键词
P53蛋白
胃肿瘤
淋巴转移灶
免疫组织化学
NM23
nucleoside diphosphate kinases
protein p53
stomach neoplasms
lymphogenous metastasis
immunohistochemistry
