摘要
目的探讨老年大鼠小肠缺血预处理对心脏缺血再灌注损伤的影响。方法在大鼠离体心脏灌注模型上，观察在体小肠缺血预处理对心脏缺血再灌注损伤的影响。结果小肠缺血预处理组和心脏缺血再灌注损伤组检测结果分别为蛋白５６．３±０．７和９１．５±１５．４ｍｇ／Ｌ，乳酸脱氢酶（ＬＤＨ）１０１．２±２９．３和２３０．６±３５．９Ｕ／Ｌ，组织蛋白酶Ｄ（ＣＤ）１．４±０．４和１．９±０．５Ｕ／ｍｇ蛋白，丙二醛（ＭＤＡ）１．２±０．２和１．６±０．４μｍｏｌ／ｇｄｗ，各指标两组比较，差异有显著性（均为Ｐ＜０．０１）。蛋白激酶Ｃ（ＰＫＣ）抑制剂组上述指标明显高于预处理组。结论老年大鼠小肠缺血预处理对心脏缺血再灌注损伤具有保护作用，这种器官间的交叉预处理保护作用涉及ＰＫＣ途径。
Objective To investigate the effect of ischemic preconditioning (IPC) in small intestine on ischemic reperfusion injury of aged rat heart. Methods In a model of isolated perfused heart from aged rats, the effects of IPC in invivo small intestine on heart ischemic reperfusion injury were observed. Results Small intestinal preconditioning reduced the leakage of protein (563±07 vs 915±154mg/L,P<001),lactate dehydrogenase (LDH, 1012±293 vs 2306±359U/L,P<001) and cathpsin D (CD, 14±04 vs 19±05U/mg pr, P<001) and lowered MDA (12±02 vs 16±04μmol/g dw,P<001) formation in myocardial tissue. Inhibitor of protein kinase C (PKC) abolished the above protective effects. Conclusions Small intestinal IPC can protect the heart of aged rats from ischemic reperfusion injury. PKC pathway might be involved in the cross protective effect of preconditioning.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
1998年第1期37-39,共3页
Chinese Journal of Geriatrics
关键词
心肌缺血
再灌注损伤
蛋白激酶C
老年人
Myocardial ischemia\ \ Myocardial reperfusion injury\ \ Protein kinase C
