期刊文献+

SSCP技术及在植物中的应用 被引量:7

Single-Strand Conformation Polymorphism and Its Application in Plant Research
在线阅读 下载PDF
导出
摘要 单链构象多态性(SSCP)技术是一种简便、灵敏的多态性检测方法,近年在植物研究中得到了较多应用。现就SSCP技术的原理、特点、影响因素以及在植物研究中的应用进行综述。 Single-Strand Conformation Polymorphism (SSCP) is a simple and sensitive method to detect polymorphisms. In recent years, this method has been widely used in plant research. In this paper, the principle, methods, characteristies and affected factors of SSCP were simply introduced. And, the applications of SSCP in plant research were reviewed.
作者 李媛媛
出处 《北方园艺》 CAS 北大核心 2009年第5期122-124,共3页 Northern Horticulture
基金 山东省中青年科学家科研奖励基金计划资助项目(2007BSA07009)
关键词 SSCP 影响因素 多样性 定位 Single strand conformation polymorphism Affected factors Diversity Mapping
  • 相关文献

参考文献32

  • 1Noumi T, Mosher M E, Natori S, et al. A phenylalanine for serine substitution in the B subunit of Escherichia coli Fl-ATPase affects dependence of its acticity on divalentication [J], J Biol Chem,1984,259:10071-10075.
  • 2Orita M, Iwahana H, Kanazawa H. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymor- phisms [J]. Proc Natl Acad Sci USA,1989,86:2766-2770.
  • 3Orita M, Suzuki Y, Sekiya T, et al. Rapid and sensitive detection of oint mutations and DNA polymorphisms using the polymerase chain reaction [J]. Genomics,1989(5) :874.
  • 4Hoshino S, Kimura A, .Fukuda Y, et al. Polymerase chain reaction- single strand conformation polymorphism analysis of potymorphism in DpAl and DPBl genes: a single, economical and rapid method for histocompatibility testing [J]. Hum Immunol, 1992,33:98.
  • 5Hayashi K, Yandell D W. How sensitive is PCR-SSCP[J]. Human Mutation, 1993(2) : 338-346.
  • 6Sheffield V C, Beck J S, Kwitek A E. The sensitivity of single strand conformation polymorphism analysis for the detection of single base substitu tions [J]. Genomics. 1993,16 : 325-332.
  • 7Vidal Puig A, Moiler D E. Comparative sensitivity of alternative single- strand conformation polymorhpism (5kSCP) Methods[J]. Bio Techniques, 1994,17:490-496.
  • 8Hongo T, Buzard G S,Calvert R J. Heterogeneity studies [J]. Human Molecular Genetics,1994(3) :471- 476.
  • 9余桂红,唐克轩,马鸿翔,吴勇华,周淼平,张旭,任丽娟,陆维忠.凝胶组成对小麦SSCP技术的影响[J].江苏农业学报,2007,23(5):391-395. 被引量:1
  • 10Dumolin L S, Bodenes C, Petit R J. Delection of rare polymorphism in mitochondrial DNA of oaks with PCR-RFL P combined to SSCP analysis [J]. Forest Genetics, 1996 ( 3 ) : 227-230.

二级参考文献34

  • 1沈银柱,刘植义,张召铎,司智海,黄占景,史兰波,孟庆昌.诱发小麦成熟胚愈伤组织及其再生植株抗盐性变异的研究[J].Acta Genetica Sinica,1993,20(3):253-261. 被引量:25
  • 2朱伟锋,蔡贵庆,黄艳梅,徐基清,区敬华,伍新尧.SNP分型前的PAGE检测[J].生物技术,2006,16(5):20-23. 被引量:3
  • 3赵瑞,刘珍珍,徐桂云,杨宁.利用PCR-SSCP分析鸡绿壳蛋基因的SNP标记[J].农业生物技术学报,2006,14(5):673-676. 被引量:12
  • 4刘佳建 彭中镇 等.甘油浓度对PCR-SSCP分辨效果的影响[J].生物技术通讯,1998,(2):160-161.
  • 5Wang X R,American J Botany,2000年
  • 6Xiang Q Y,Amer J Bot,1998年,85卷,285页
  • 7Lu M Z,Heredity,1995年,74期,582页
  • 8Raizada,J,Kumar,S.Ahuja,P.S.Cloning and characterization of polyphenol oxidase gene in Camellia sinensis(L.)O.kuntze.http://www.ncbi.nlm.nih,gov,2004.
  • 9Joseph Sambrook,David W.Russell.Molecular cloning.Third edition.Cold Spring Harbor Lab Press,2002,418-425.
  • 10W.Castelblanco,M.Fregene.SSCP-SNP-based conserved ortholog set(COS)markers for comparative genomics in cassava (Manihot esculenta) Crantz.plant Molecular Bi ology Reporter,2006,24(2):229-236.

共引文献13

同被引文献114

引证文献7

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部