摘要
目的:克隆与分析大鼠不同剪切的Lims基因。方法:应用巢式RT-PCR,以大鼠cDNA为模板,扩增Lims基因不同剪切子,构入PinPointTM Xa-1T质粒,测序鉴定。结果:测序表明克隆了一种新的Lims基因变异剪切体Lims E,编码区为1164bp,编码387个氨基酸。结论:比较基因组学分析显示,成功地克隆了一个新的大鼠Lims基因剪切子Lims E,为进一步研究Lims基因在细胞发育中的功能打下了基础。
Objective： To clone and analyze the different splicing variants ofLims gene in rats. Methods： Splicing variants of Lims gene were amplified by RT-PCR from rat cDNA and inserted into PinPointTM Xa-IT vector. Two position clones selected by PCR were sequenced. Results： A novel splicing variantsLims E has been cloned. Liras E contains an 1164 bp open reading frame （ORF） which en- codes a 387 amino acids （AAs） protein. Conclusions： Comparative genorne analysis shows that Lims E, a novel splicing variant of Lims gene, has been cloned in rats and it establishes a foundation for further study of the function of Lims genes in cell development.
出处
《现代生物医学进展》
CAS
2009年第9期1620-1623,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金资助项目(30000088)
