摘要
[目的]探究Na+-Ti(SO4)2法检测鲫鱼中孔雀石绿残留的方法。[方法]将孔雀石绿溶于浓硫酸显黄色,稀释后显暗黄色,用Ti(SO4)2使其褪色,加入Na+变为白色沉淀,依据沉淀量对孔雀石绿残留进行定量测量并采用正交实验优化实验条件。[结果]采用pH值为7.4、硫酸钛和氯化钠的浓度均为2.0 mol/L时,可有效地检测孔雀石绿残留。该方法最低检测限为0.01 mg/kg,在鲫鱼肌肉和肝脏中的回收率分别为86.63%~91.31%和76.63%~78.08%,相对标准偏差分别为4.21%~2.19%和3.93%~1.86%。孔雀石绿残留在药浴1 h后鲫鱼肝脏中能检测到的含量最高(0.033 2 mg);到6 h时再次出现检测峰值(0.0310 mg),之后随鲫鱼暂养时间的延长检测量显著下降,到第30天肌肉和肝脏中均仅能检测到0.0001 mg。[结论]该方法实验成本低,操作简单,周期短,适用于检测水产品中孔雀石绿残留。
[Objective] The aim was to research the method of determining malachite green residue in crucian carp tissues by Na^＋-Ti（ SO4）2. [Method]
The malachite green was dissolved in the concentrated sulfuric acid and displayed yellow, which was diluted and then displayed dark yellow.The malachite green was faded with Ti （ SO4 ）2 and was changed into white precipitate after adding the Na^＋ According to quantity of precipitate, the malachite green residue was determined quantitativly and the orthogonal test was used to optimize the experimental conditions. [Result] The malachite green residue was measured effectively when pH value was 7.4 and the titanium sulfate and sodium chloride were all 2.0 mol/L.The lowest limit of detection of this method was 0.01mg/kg, its recovery rate in the muscle and liver of crucian carp were 86.63% - 91.31% ,76.63% - 78.08% resp. and the relative standard deviation was 4.21%-2.19% and 3.93% -1.86% resp.The content of malachite green residue was highest （0.033 2 mg） when it was detected in the
liver of crucian carp after bathing 1h with drug, until 6h the testing quantity peak （0.0310 mg） appeared again, then the testing quantity was decreased remarkably with the prolonging of temporary rearing time, which was tested by 0.000 1 mg in the muscle and liver until 30 d. [Conclusion] This method was low in experiment cost, simple in manipulation, short in cycle and it was much suitable to test the malachite green residue in the aquatic pmducts.
出处
《安徽农业科学》
CAS
北大核心
2009年第12期5347-5349,共3页
Journal of Anhui Agricultural Sciences
基金
水产品中主要禁用药物快速检测新技术的研究(6660-0647)
