摘要
从江苏省苏北地区６市的不同类型土壤采集土样４２２份，分离出苏云金杆菌７６株。经形态学观察、生理生化反应及血清学鉴定，认为它们分别属于苏云金杆菌库斯塔克亚种，血清型Ｈ３（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｋｕｒｓｔａｋｉ，ｓｅｒｏｔｙｐｅＨ３）；鲇泽亚种，血清型Ｈ７（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ａｉｚａｗａｉ，ｓｅｒｏｔｙｐｅＨ７）；达姆斯塔特亚种，血清型Ｈ１０（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｄａｒｍｓｔａｄｉｅｎｓｉｓ，ｓｅｒｏｔｙｐｅＨ１０）；科尔默亚种，血清型Ｈ２１（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｃｏｌｍｅｒｉ，ｓｅｒｏｔｙｐｅＨ２１）和贵阳亚种，血清型Ｈ４３（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｇｕｉｙａｎｇｉｅｎｓｉｓ，ｓｅｒｏｔｙｐｅＨ４３）；另有１株自凝菌（ａｕｔｏ－ａｇｇｌｕｔｉｎａｔｅ）。苏北地区出菌土样率为１５．２％，出菌率为１８％，优势血清型为Ｈ３、Ｈ１０和Ｈ２１。还讨论了不同类型植被和不同ｐＨ值土壤中苏云金杆菌的分布特点。通过对７６个分离株的毒力测定，筛选出４个对赤松毛虫、小菜蛾、玉米?
According to the different sorts of soils,76 Bt isolates of Bacillus thuringiensis were isolated in 422 soil samples from the North part of Jiangsu Province.The results of morphological characters,biochemical reactions and Hserotype identification indicated that these isolates belonged to Bacillus thuringiensis subsp.kurstaki,serotype H3;Bacillus thuringiensis subsp.aizawai,serotype H7;Bacillus thuringiensis subsp.darmstadiensis,serotype H10;Bacillus thuringiensis subsp.colmeri,serotype H21;Bacillus thuringiensis subsp.guiyangiensis,serotype H43 and autoagglutinate,respectively.The rate of soils sample with Bt and the rate of Bt isolates in the North part of Jiangsu Province were 152% and 18%,respectively.Predominant serotypes were H3,H10 and H21.The distribution character of Bt in different pH value and plant covers of varioussoilshadalsobeendiscussed.The results of bioassay showed that four isolates (especially Ly3032 and Js2101 strain) had high toxicity to such major forest and crop pests as Dendrolimus spectabilis,Plutlles xylostells and Ostrinin furnacalis.They will supply important resource of Bt strain for developing high toxicity insecticides.
出处
《江苏农业学报》
CSCD
北大核心
1998年第2期96-102,共7页
Jiangsu Journal of Agricultural Sciences
基金
江苏省应用基础研究课题
关键词
苏云金杆菌
鞭毛血清型
杀虫特性
Bacillus thuringiensis
Hserotype
larvicidal character.
