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两种真核蛋氨酸裂解酶基因克隆、鉴定及分析比较 被引量：5

Cloning and analysing of Two eukaryotic Methionine-Lyase Genes
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摘要 目的:克隆并分析从阴道毛滴虫中得到两种蛋氨酸裂解酶同工酶基因(TVmgl1,TVmgl2)。方法:提取阴道毛滴虫总RNA,由逆转录获取cDNA,PCR大量扩增得到TVmgl1和TVmgl2基因,分离纯化后,连接pMD-19-T克隆载体,转菌后筛选得到阳性克隆,测序得到碱基序列。结果:序列分析所得基因,序列TVmgl1为1191bp,TVmglL2为1197bp,其编码氨基酸序列TVMGL1与TVMGL2有69%的同源性。结论:克隆得到TVmgl1、TVmgl2基因。 Objective： To colone, analyse the structure of the two genes encoding methionionase from Trichomonas vaginalis . Me.theds： Total RNA was extracted from primitive protozoon Trichomonas vaginalis,cDNA fragments encoding Methionine γ- lyase genes were amplified by RT-PCR using specific primers,and then cloned into pMD-19-T vector.The two inserted Methioninase genes were sequenced . Results： Analysis indicates that the DNA fragments are 1191bp and 1197bp in length and have 69% high nucleotide homology with that described in GenBank. Conclusion： Two L- Methioninc γ-lyasc isozyme genes were successfully cloned and identified from Triehomonas vaginalis.
作者 林琳 赵瑜 付崴 唐金玲 彭江丽 张晓芳 田长富
机构地区 昆明医学院生物医学工程研究中心基因与蛋白质工程研究室
出处 《中国民族民间医药》 2009年第7期1-3,6,共4页 Chinese Journal of Ethnomedicine and Ethnopharmacy
基金 国家自然科学基金30760287 云南省科技厅基金2006C0044M 云南省教育厅基金5Z0322C
关键词 蛋氨酸裂解酶 基因克隆 阴道毛滴虫 L-Methionine γ-lyase Gene cloning Trichomonas vaginalis
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献10

  • 1Cellarier E, Durando X, Vasson MP,et al. Methionine dependency and cancer treatment [J] .Cancer Treat Rev,2003,29 (6) :489-499.PMID: 14585259.
  • 2Takakura T, Takimoto A, Notsu Y, et al.Physieochemical and pharmacokinetic characterization of highly potent recombinant L-methionine gamma-lyase conjugated with polyethylene glycol as an antitumor agent [J] .Cancer Res.2006,66 (5) :2807-14. PMID: 16510603.
  • 3Gupta A, Miki K, Xu M, et al. Combination efficacy of doxorubicin and adenoviral methioninase gene therapy with prodrug selenomethionine [J] .Anticancer Res. 2003,23:1181-8. PMID: 12820369.
  • 4Zhao R,Domann FE,Zhong W.Apoptosis induced by selenomethioninc and methioninase is superoxide mediated and p53-dependent in huroan prostate cancer cells [J] .Mol Cancer Thor, 2006, 5 (12) :3275-3284.PMID:17172431.
  • 5Yamamoto N, Gupta A, Xu M, et al. Methioninase gene therapy with selenomethionine induces apoptosis in bcl-2-overproducing lung cancer cells [J] . Cancer Gene Ther. 2003,10:445-50. PMID:12768189.
  • 6Kim A, Oh JH, Park JM, et al. Methylselenol generated from selenomethionine by methioninase downregulates integrin expression and induces caspase-mediated apoptosis of B16F10 melanoma cells [J] . Cell Physiol. 2007,212 (2) 386-400. PMID: 17348006.
  • 7Miki K, AI-Refaie W, Xu M, et al. Methioninase gene therapy of human cancer cells is synergistic with recombinant methioninase treatment [J] .Cancer res, 2000,60 (10) :2696-2702. PMID:10825143.
  • 8Lu S, Hoestje SM, Choo EM, et al. Methionine restriction induces apoptosis of prostate cancer cells via the c-Jun N-terminal kinase-mediated signaling pathway [J] . Cancer Lett, 2002, 179 (1): 51-58.
  • 9马百坤,唐晓莹,黄惠臣,时永辉.蛋氨酸裂解酶的基因克隆及序列分析[J].医学研究生学报,2006,19(12):1063-1066. 被引量：10
  • 10McKie AE, Edlind T, Walker J,et al. The primitive protozoon Tfichomonas vaginalis contains two methionine gamma-lyase genes that encode members of the gamma-family of pyridoxal 5'-phosphate-dependent enzymes [J] .Biol Chem,

二级参考文献7

  • 1徐振宇,高建平,张征宇,葛京平,许传亮,孙颖浩.RECK基因在前列腺癌组织中的表达及其与基质金属蛋白酶的关系[J].医学研究生学报,2005,18(7):577-579. 被引量：6
  • 2Miki K,Xu M,An Z,et al.Survival efficacy of the combination of the methioninase gene and methioninase in a lung cancer orthotopic model[J].Cancer Gene Ther,2000,7 (2):332-338.
  • 3Cellarier E,Durando X,Vasson MP,et al.Methionine dependency and cancer treatment[J].Cancer Treat Rev,2003,29 (6):489-499.
  • 4Yamamoto N,Gupta A,Xu M,et al.Methioninase gene therapy with selenomethionine induces apoptosis in bcl-2-overproducing lung cancer cells[J].Cancer Gene Ther,2003,10(6):445-450.
  • 5Kokkinakis DM,Wick JB,Zhou QX.Metabolic response of normal and malignant tissue to acute and chronic methionine stress in athymic mice bearing human glial tumor xenografts[J].Chem Res Toxicol,2002,15 (11):1472-1479.
  • 6Kokkinakis DM.Methionine-stress:a pleiotropic approach in enhancing the efficacy of chemotherapy[J].Cancer Lett,2006,233(2):195-207.
  • 7Peron K,Jones TN,Gauthier SA,et al.Targeting of a novel fusion protein containing methioninase to the urokinase receptor to inhibit breast cancer cell migration and proliferation[J].Cancer Chemother Pharmacol,2003,52 (4):270-276.

共引文献9

同被引文献50

  • 1田腊梅,任小丽,胡振良,曾红静,田长富.2种重组真核蛋氨酸酶原核体系表达及活性比较[J].昆明医科大学学报,2012,33(5):11-14. 被引量：1
  • 2马百坤,唐晓莹,黄惠臣,时永辉.蛋氨酸裂解酶的基因克隆及序列分析[J].医学研究生学报,2006,19(12):1063-1066. 被引量：10
  • 3Tanaka H, Esaki N, Soda K. Properties of L-methionine γ-lyase from Pseudomonas ovalis. Biochemistry, 1977,16( 1 ) : 100-106.
  • 4Nakayama T, Esaki N, Lee W J, et al. Purification and properties of L-methionine γ-lyase from Aerorrvonas sp. Agric Biol Chem, 1984, 48(9) :2367-2369.
  • 5Miki K, Xu M, An Z, et al. Survival efficacy of the combinalion of the methioninase gene and methioninase in a lung cancer orthotopie model. Cancer Gene Ther, 2000,7 (2) :332-338.
  • 6Tan Y, Sr Zavala J, Han Q, et al. Recombinant methioninase infusion reduces the biochemical endpoint of serummethionine with minimal toxicity in high-stage cancer patients. Anticancer Res, 1997, 17(5B) :3857-3860.
  • 7Miki K, Al-Refaie W, Xu M, et al. Methioninase gene therapy of human cancer cells is synergistic with recombinant methioninase treatment. Cancer Res, 2000, 60(10) :2696-2702.
  • 8Chan E C, Chang P Y, Wu J T, et al. Enzymatic Assay of Homocysteine on Microtiter Plates or a TECAN Analyzer Using Crude Lysate Containing Rocombinant Methionine γ-Lyase. Annals of Clinical & Laboratory Science, 2005, 35(2) : 155-161.
  • 9MeKie A E, Edlind T, Walker J, et al. The primitive protozoon Trichomonas vaginalis contains two methionine-lyase genes that encode members of the-family of pyridoxal 5- phosphate-dependent enzymes. Biol Chem, 1998, 273 ([0) :5549-5556.
  • 10Carlton J C, Hirt R P, Silva J C, et al. Draft Genome Sequence of the Sexually Transmitted Pathogen Triehomonas vaginalis. Science,2007, 315(5809) :207-212.

引证文献5

二级引证文献6

中国民族民间医药
2009年 第7期

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