摘要
以脂肪酶Novozym-435催化葡萄糖和10-十一碳烯酸合成了6-O-(10-十一碳烯酸)-葡萄糖酯,在K2S2O8引发下合成了葡萄糖基聚合物。采用复凝聚法以葡萄糖基聚合物和海藻酸钠为基质材料,将非诺洛芬钙包裹制成缓释微球。通过L9(34)正交实验得出微球的最佳制备工艺条件,结果是葡萄糖基聚合物:海藻酸钠质量比=1:2,pH3.0,搅拌速度400r/min,反应成球温度45℃。在最佳工艺条件下制备的非诺洛芬钙缓释微球,粒径范围是10～20μm,平均药物包封率(73.74±3.12)%。同时,体外溶出试验表明,该微球具有较好的缓释作用。
After immobilized lipase enzyme Novozym-435 catalyzed glucose and undecylenic to regioselecuve synthesis 6-O-（10-undecylenoyl） D-glucose, the glucose-based polymer were prepared using K2S208 as a chemical initiator. The suitable sustained-release microspheres containing fenoprofen calcium were prepared using glucose-based polymers and sodium alginate as base material. The L9（3^4） orthogonal experimental design to optimize the preparation procedure of the microspheres. The result showed that the mass ratio of polymer arid alginate was 1：2, pH 3.0, the rotate speed was 400 r/min, and the temperature was 45℃ is the best preparation condition. The particle size range of the microspheres is 10-20μm. The average drug envelopment is （73.74±3.12）%.The release in vitro of the microspheres indicated that these microspheres may prove better sustained release of drugs.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2009年第2期321-325,共5页
Genomics and Applied Biology
基金
国家自然科学基金(20366002-2)
广西科技创新能力与条件建设项目(桂能科0630006-5B)资助
关键词
葡萄糖基聚合物
酶催化合成
微球
缓释制剂
Glucose-based polymers, Enzyme-catalyzed synthesis, Microspheres, Sustained-release procedures
