摘要
目的探讨GPⅠbαKozak-5T/C基因多态性与冠心病相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析检测208例宁夏回族人群(冠心病组96例,对照组112例)Kozak-5T/C基因型及等位基因频率。结果208例宁夏回族人群GPⅠbαKozak-5T/C基因CC、CT和TT三种基因型分布频率分别为8.6%、23.1%和68.3%,C和T等位基因的分布频率分别为20.2%和79.8%。112例对照组CC、CT和TT三种基因型的分布频率分别为8.9%、23.2%和67.9%,C和T等位基因的分布频率分别为20.5%和79.5%;96例冠心病组的CC、CT和TT三种基因型的分布频率分别为8.3%、22.9%和68.8%,C和T等位基因的分布频率分别为19.8%和80.2%。GPⅠbαKozak-5T/C基因多态性在冠心病组及对照组之间的分布差异无统计学意义(χ21=0.029,P1=0.986;χ22=0.036,P2=0.851)。结论GPⅠbαKozak序列-5T/C基因多态性与冠心病的发生未见相关性。
Objective To explore the correlation between polymorphism of platelet glycoprotein IbαKozak-5T/C （GPIbα） gene and coronary heart disease. Methods Ninety-six patients with angiographically confirmed coronary heart disease and one-hundred and twelve controls were genotyped for the Kozak-5T/Cpolymorphism by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis. Results No statistical difference in genotype frequency or allele frequency of GP I bagene between cases and controls was found （χ^21 = 0. 029, P1 = 0.986;χ^22= 0.036, P2 = 0.851 ）. Conclusion Our findings indicate that no association exists between Polymorphism of Platelet Glycoprotein I bαKozak -ST/C Gene and coronary heart disease in Hui Nationality population.
出处
《宁夏医科大学学报》
2009年第2期183-185,共3页
Journal of Ningxia Medical University
