摘要
利用分子生物学技术构建人源β-防御素-2(HBD-2)真核表达重组质粒pcDNA3.1-zeo(+)-HBD-2,经脂质体介导转染293细胞,RT-PCR检测到HBD-2基因成功转染293细胞。收集培养上清,采用肉汤对倍稀释法检测抗菌活性,结果显示培养上清对金黄色葡萄球菌有抗菌活性,而在相同条件下未检测到抗铜绿假单胞菌活性,提示人源β-防御素-2(HBD-2)可能具有优势抗金黄色葡萄球菌活性。
In this study, HBD-2 eukaryotic expression recombinant plasmid peDNA 3.1-zeo（＋）-HBD-2 was constructed by the molecular cloning technologies, and 293 cells were transfeeted by liposome. The cell culture supernatant was collected and its antibacterial activities against Staphylococcus aureus ATCC25923 and Pseudomanas aeruginosa ATCC27853 were tested. The antibacterial activity against S. aureus of the culture supernatant of the HBD-2 gene transfeeted-293 cells was detected, whereas in the same condition, the antibacterial activity against P. aeruginosa was not detected. This result suggested that HBD-2 may preferentially kill Staphylococcus aureus.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2009年第2期371-373,共3页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30472176)
关键词
Β-防御素-2
抗金色葡萄球菌活性
293细胞
Human beta-defensin-2（HBD-2） Antibacterial activity against S. aureus 293 cells
