摘要
目的:研究白藜芦醇(Res)对U251人脑胶质瘤细胞生长的抑制及相关作用机制。方法:将不同浓度Res作用于U251细胞系,MTT法检测Res对U251细胞生长增值的抑制作用,流式细胞仪(FCM)检测Res.对细胞凋亡的影响,免疫组织化学法检测Res处理前后Bcl-2,Bcl-XL,CyclinD1,STAT3的表达及Western blot(蛋白印迹法)法检测Bcl-2,Bcl-XL,Cy-clinD1,Caspase-3,Bax,STAT3的表达。结果:经Res处理后的U251细胞,MTT结果显示细胞增殖现象被抑制,并呈时间-剂量依赖性;FCM显示随着Res作用时间的延长,细胞晚期凋亡数增加;免疫组织化学法显示Bcl-2,Bcl-XL,CyclinD1,STAT3的表达下降;Western-blot检测显示Bcl-2,Bcl-XL,CyclinD1,STAT3表达下降,而Caspase-3和Bax的表达上调。结论:Res诱导U251细胞凋亡的分子机制可能是通过下调Bcl-2,Bcl-XL蛋白的表达,而上调Bax与Caspase-3蛋白的表达,并随着Res剂量与作用时间的延长这种趋势在加强。
Objective： To investigate the suppressive effect of resveratrol on growth of U251 human glioma cells and its correlated mechanism. Method： U251 human glioma cells were treated with resveratrol at various concentrations, MTT assay was used to determine the inhibitory rate of cell proliferation, FCM to detect the cell apoptosis, the expressions of Bcl-2, Bcl-XL, STAT3 and CyclinD1 were analysed by immunohistochemistry and Western blot to examine the expression of Bcl-2, Bcl-XL, STAT3, CyclinD1, Caspase-3 and Bax. Result： After treatment with resveratrol, MTT assay showed the growth of U251 cells was inhibited in dose-dependent and time-dependent manners, apoptosis of cells advanced stage was built up, immunohistochemical staining displayed decreased the expression of Bcl-2, Bcl- XL, STAT3 and CyclinD1 and Western blot showed that resveratrol decreased the expression of Bcl-2, Bcl-XL, STAT3 and CyclinD1, and built up Bax and Caspase-3. Conclusion： It is possible that downregulated the expression of Bcl-2, Bcl-XL, but upregulated Bax and Caspase-3, and the indication was obviously in dose-dependent and time-dependent manners.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第8期1027-1031,共5页
China Journal of Chinese Materia Medica
基金
天津市卫生局研究项目(2005092)
