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RNAi抑制卵巢癌SKOV_3细胞MMP-24基因表达及对细胞侵袭的影响

Inhibitory effects of RNA interference on MMP-24 expression and invasiveness of ovarian cancer SKOV_3 cells
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摘要 目的探讨应用RNA干扰技术沉默MMP-24基因表达,研究其对人卵巢上皮癌SKOV3细胞侵袭力的影响。方法设计合成2条特异性针对MMP-24基因的siRNA,转染至SKOV3中,采用RT-PCR和Western blotting检测MMP-24 mRNA和蛋白表达情况,细胞侵袭实验观察转染后细胞侵袭能力的变化。结果转染siRNA后,卵巢癌细胞MMP-24 mRNA表达受抑制,蛋白表达降低,细胞侵袭力减弱。结论RNAi沉默MMP-24基因可影响卵巢癌细胞的侵袭,有可能成为治疗卵巢癌的新途径。 Objective To investigate the inhibitory effect of RNA interference （RNAi） on MMP-24 expression and invasiveness of ovarian cancer SKOV3 cells. Methods Two pairs of small interfering RNA （siRNA） specific to MMP-24 mRNA were designed and transfected into SKOV3 cells. RT-PCR and Western blotting were used to detect the mRNA and protein expressions of MMP-24, and the cell invasiveness was assessed using an in vitro invasion test. Results After transfection with siRNA, the mRNA and protein expression levels of MMP-24 were obviously reduced in SKOV3 cells, which also showed significantly decreased invasiveness in vitro. Condusion MMP-24 gene silencing by RNAi can suppress the invasiveness of ovarian cancer SKOV3cells in vitro,which may provide a new therapeutic approach of ovarian cancer.
作者 骆亚平 钟梅 汪丽萍 孙桂芹 黎静
机构地区 南方医科大学南方医院妇产科
出处 《南方医科大学学报》 CAS CSCD 北大核心 2009年第4期781-784,共4页 Journal of Southern Medical University
关键词 RNA干扰 MMP-24卵巢肿瘤 侵袭 RNA interference MMP-24 ovarian neoplasms invasiveness
分类号 R737.31 [医药卫生—肿瘤]
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参考文献10

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二级参考文献8

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共引文献8

南方医科大学学报
2009年 第4期

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