摘要
目的探讨槲皮素治疗糖尿病肾病机制。方法对链脲佐菌素诱发糖尿病肾病大鼠给予槲皮素１００ｍｇ·ｋｇ－１·ｄ－１治疗９周，测定糖尿病大鼠体重、肾重、血糖、胰岛素、果糖胺、脂质过氧化物（ＬＰＯ）及超氧化物歧化酶（ＳＯＤ）改变，同时测定肾脏皮质组织ＬＰＯ、果糖胺及晚期糖化终产物（ＡＧＥｓ）、糖化产物（ｐｅｎｔｏｓｉｄｉｎｅ）及脂质过氧化物加合物荧光，并观察尿白蛋白排泄量及肾脏组织病理改变。结果槲皮素对早期糖化产物果糖胺无抑制作用，但对肾脏组织上ＬＰＯ及ＡＧＥｓ、ｐｅｎｔｏｓｉｄｉｎｅ及脂质过氧化物加合荧光均有明显抑制作用，尿白蛋白排泄量明显减少，肾小球基底膜和基质增生均有改善。结论槲皮素通过抑制糖尿病大鼠肾脏组织非酶糖化及氧化。
Objective To investigate the inhibitory effects of Quercetin on diabetic nephropathy. Methods Quercetin 100mg.kg -1 .d -1 , was given to streptozotocin induced diabetic rats. Rats were killed after 9 weeks treatment. Body weight, kidney weight, blood glucose, insulin, LPO, fructosamine and RBC SOD were measured. We also measured LPO、 fructosamine、 the fluorescence intensities of AGEs, pentosidine, lipoperoxide adducts in renal cortex. Results The early non enzymatic glycation products fructosamine were not inhibited in Quercetin treated group, however, LPO and the fluorescence intensities of AGEs, pentosidine, and MDA and HNE adducts in renal cortex were significantly reduced in Quercetin treated gronp than untreated DM group. The urinary albuminuria excretion in Quercetin group was significantly decreased than untreated DM group. Glomerular basement membrane thickening and mesangial matrix expansion were improved in the treated group. Conclusion The results suggest that Quercetin may inhibite non enzymatic glycation and oxidation in the kidney of streptozotocin induced diabetic rats and control the diabetic nephropathy.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1998年第1期34-37,共4页
Chinese Journal of Endocrinology and Metabolism
关键词
糖尿病肾病
糖尿病
非酶糖化
槲皮素
药物疗法
Diabetic nephropathy Nonenzymatic glycation Glycoxidation Quercetin Aminoguanidine
