摘要
目的为鉴定伤寒沙门菌的耐药性获得与整合子之间的关系,确定该菌抗性基因扩散的可能机制。方法我们选取32株多药耐药的伤寒沙门菌株提取全基因组DNA,扩增整合子,并以此作为模板检测抗性基因。结果以伤寒沙门菌基因组DNA为模板有81.2%的菌株,能够扩增出相应整合子的目的片段。具有四环素抗性、氨苄青霉素抗性和复方磺胺抗性的样品,以其扩增产物作为模板全部能扩增出相应抗性基因suⅡ、blaTEM-1和tetG。结论伤寒沙门菌的抗性基因是整合在Ⅰ类整合子中的,故整合子的转移应是伤寒沙门菌抗性基因传递的一种主要途径。
Objective To explore the relation of Salmonella typhi resistance and integron cassettes and discover the potential mechanism of resistance gene transferring in Salmonella typhi. Methods Thirty-two stains of Salmonella typhi genome DNA were extracted and the integron were amplified by PCR, su Ⅱ ,blaTEM-1 and tetG gene were subsequently detected by PCR with the integron amplified products as template. Results The integron Ⅰ has been detected in 81.2 percent of resistance stains. The gene su Ⅱ , blaTEM-l and tetG were detected, in strains of Amp^ ＋ , Tet^ ＋ and SMZ ^＋ , with the their ingeron amplified products. Conclusion It suggested that the resistance genes located in the integron Ⅰ in Salmonella typhi and integron transferring should be a major way in the resistance acquisition of Salmonella typhi.
出处
《中国实验诊断学》
北大核心
2009年第4期466-468,共3页
Chinese Journal of Laboratory Diagnosis
基金
贵州省优秀科技教育人才省长专项基金资助
关键词
伤寒沙门菌
多药耐药
抗性基因
整合子
Salmonella Typhi
multidrug resistance
resistant gene
integron
