摘要
为了研究阵发性睡眠性血红蛋白尿(PNH)患者的淋巴细胞对红系和粒-巨噬系祖细胞的影响,将PNH患者的植物血凝素-淋巴细胞培养液(PHA-LCM)加入造血祖细胞培养基中,用于红系爆式集落形成单位(BFU-E)和粒-巨噬系集落形成单位(CFU-GM)的培养。结果在含有PNH患者的PHA-LCM的培养条件下,PNH患者骨髓BFU-E和CFU-GM集落数明显低于含有正常人PHA-LCM的培养条件下的集落数;正常人骨髓BFU-E和CFU-GM集落数亦明显低于含有正常人的PHA-LCM培养条件下的集落数。结果表明PNH患者淋巴细胞对造血的支持作用减弱。
The purpose of this study was to investigate the effects of lymphocytes from patients with paroxysmal nocturnal hemoglobinuria(PNH)on erythroid and myeloid progenitors.Phytohaemaglutinin-stimulating lymphocytes conditioned medium(PNA-LCM)from PNH blood was added to the culture medium for the culture of PNH and normal burst-forming units-erythroid(BFU-E)and colony-forming units——granulocyte/macrophage(CFU-GM).The results showed that the number of PNH bone marrow BFU-E and CFU-GM in the medium containing PHA-LCM from PNH blood was smaller than that form normal blood;that the number of normal bone marrow BFU-E and CFU-GM grown in the medium containing PHA-LCM from PNH blood decreased more than that from normal blood.It was suggested that diminished number of PNH bone marrow BFU-E and CFU-GM resulted in declined supporting effects of lymphocytes on hematopoiesis.
作者
高清平
杨卫泽
陈济民
李秀珍
梅广义
陈友华
夏虹
王巧玲
Gao Qingping;Yang Weize;Chen Jimin(Department of Hematology,First Affiliated Hospital,Hubei Medical University,Wuhan 430060,China)
