摘要
目的探讨海洛因对发育中仔鼠大脑颞叶皮层结构及腺苷脱氨酶（ADA）、琥珀酸脱氢酶（SDH）、一氧化氮合酶（NOS）及还原型谷胱甘肽（GSH）活性的影响。方法对48例受孕小鼠（第8天开始）分别连续注射3种不同浓度（1.0g/L、1.5g/L和2.0g/L）的海洛因溶液和等量的生理盐水至小鼠分娩,称量检测各实验组仔鼠在胚胎15d（E15）及生后1d、7d、15d时大脑重量的变化,用生物显微技术观察仔鼠大脑颞叶皮层结构的变化,用比色法检测仔鼠大脑ADA、SDH、NOS、GSH的活性变化。结果海洛因影响仔鼠脑的发育,实验组仔鼠大脑重量明显低于对照组;实验组仔鼠大脑颞叶皮层结构不清,神经元发育不良,皮层厚度减少,除在E15和生后1d大脑颞叶皮层第Ⅵ层神经元细胞数量高于对照组外,其他发育期各层神经元细胞数量均小于对照组;1.0g/L、1.5g/L和2.0g/L3个剂量组在E15时,胎鼠大脑中ADA、SDH及NOS活性高于对照组,差异显著或极显著（P〈0.05或P〈0.01）,GSH活性低于对照组,差异极显著（P〈0.01）;仔鼠在生后15d的发育过程中,1.0g/L剂量组仔鼠大脑中ADA、SDH、NOS及GSH的活性逐渐恢复到正常水平;1.5g/L剂量组大脑中NOS和SDH的活性逐渐恢复到正常水平,但ADA的活性仍高于对照组,GSH的活性仍低于对照组;2.0g/L剂量组仔鼠大脑中ADA、SDH、NOS的活性在生后15d时仍保持较高的水平,GSH的活性仍低于对照组。双因素方差分析表明,剂量因素对结果的影响大于时间因素。结论海洛因影响发育期仔鼠大脑的重量和组织结构,这可能与脑内ADA、SDH、NOS活性升高和GSH活性下降有一定的相关性。
Objective To study the effects of heroin on the structure of cerebral temporal lobe cortex and activities of adenosine deaminase （ADA）,succinate dehydrogenase （SDH）,nitric oxide synthase （NOS） and glutathione （GSH） of developmental filial mice. Methods Forty-eight pregnant mice at the eighth day began to be continuously injected with three different concentrations （1.0g/L,1.5g/L and 2.0g/L） of heroin and the same amount of saline until the birth of filial mice. Cerebral weight of filial mice was weighted. And it was observed that the changes of the cerebral temporal lobe cortex of filial mice by bio-microscopy,and detected that the activities of ADA,SDH,NOS and GSH by colorimetry at embryo 15 days and postnatal ages of 1 day,7 days,15 days from all experimental groups. Results The development of filial cerebral was affected by heroin. The cerebral weight of experimental group was significantly lighter than that of the control group. The structure of filial cerebral temporal lobe of experiment group was not clear. The thickness of filial cerebral decreased and the number of neurons reduced except those in the cerebral temporal lobe cortex Ⅵ at embryo 15 days and postnatal 1 day. Compared with the control group,ADA,SDH and NOS activities in brain increased while GSH activity decreased significantly at embryo 15 days after administration of heroin （P〈0.01 or P〈0.05）,but the recovery of these activities at postnatal 15 days varied with different doses. All of these activities subsequently recovered to the normal level slowly in heroin administration （1.0g/L）;Activities of NOS and SDH also recovered to the normal level while others remained significantly affected at the dose of 1.5g/L;ADA,SDH and NOS activities were still higher and GSH activity was lower than that of the control in 2.0g/L group. Results of Two-Way ANOVA indicated that dose contributed more to heroin-induced brain damage than treatment time. Conclusion Heroin affects the brain weight and the structure of filial mice＇s brains. This effects might be correlated with the high activities of ADA,SDH,NOS and low activity of GSH in mice＇s brain.
出处
《解剖学报》
CAS
CSCD
北大核心
2009年第2期175-182,共8页
Acta Anatomica Sinica
基金
国家自然科学基金资助(30370163)
甘肃省自然科学基金资助(ZS031-A25-005-Z)
甘肃省创新人才基金资助(ZS006-016)
