摘要
目的:制定猫豆药材的质量标准,为该药用植物资源的开发利用提供科学依据。方法:采用了薄层色谱法、HPLC法、水分测定法、灰分测定法及浸出物测定法。结果:猫豆药材含的左旋多巴薄层色谱中,在与对照品同一位置显相同颜色的斑点。HPLC定量分析中,左旋多巴线性范围为26.45～132.25μg/mL(r=0.9992),平均回收率为103.8%,RSD=1.85%。结论:所建立的分析方法简便、准确、可靠,重现性好,可作为该药材的质量标准。
Objective ： To provide scientific basis for the utilization and development of Mucuna prurierts vat. utilis by establishing its quality control standard. Methods： The bioactive constituents were analyzed by TLC and HPLC. Moisture, ash and the extracts of Mucuna pruriens var. utilis were all determined. Results ： The TLC spots of levodopa had similar color with the control group at the same position. The results of HPLC quantitative analysis showed that the linear range of levodopa was 26.45 - 132. 25 μg/mL, r = 0. 9992, and the average recovery rate was 103.8% , RSD = 1.85%. Conclusions：This method is convenient, accurate, reliable with good reproducibility, so it can be used to establish quality standard ＇for the medicinal material.
出处
《中药材》
CAS
CSCD
北大核心
2009年第3期333-335,共3页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金资助项目(30760309)
广西科技项目(桂科攻0424008-11
桂科自0832014Z)
