摘要
对采自辽宁省部分地区瓜类保护地的36份土壤样品进行镰孢菌(Fusarium)分离培养,共获得112株镰孢菌,采用传统形态学分类和现代分子生物学方法,确定属于11个种.对其中25株镰孢菌及3株对照镰孢菌进行了通用引物PCR(UP-PCR)多样性分析.结果表明:6条引物扩增出73条带,其中多态性条带66条,占总条带数的90.4%.对供试菌株进行UP-PCR聚类图谱分析,当相似系数为0.736时,可将其划分为8个类群,其中14株尖孢镰孢菌聚为一类.UP-PCR分析体现了镰孢菌菌株间的亲缘关系及差异性,可以作为镰孢菌分类的辅助方法.
A total of 112 Fusarium isolates were obtained from 36 soil samples collected from the greenhouse melon fields of Liaoning Province, among which, 11 species were identified by traditional morphological classification and rDNA sequence analysis. Universally Primed PCR （UPPCR） was conducted to analyze the 25 strains of test Fusarium isolates and 3 strains of positive control Fusarium isolates. The results indicated that a total of 73 bands appeared after amplification by using 6 primers, and 66 bands （90.4%） were polymorphic. The isolates were clustered into eight groups at the similarity of 0. 736 by cluster analysis, among which, 14 isolates were clustered into one group. It was concluded that UP-PCR could present the genetic relationship and difference among Fusarium strains, being able to be used as an assistant method for Fusarium classification.
出处
《应用生态学报》
CAS
CSCD
北大核心
2009年第4期857-862,共6页
Chinese Journal of Applied Ecology
基金
辽宁省科技攻关计划项目(2006215004)
辽宁省自然科学基金项目(20062018)资助
关键词
镰孢菌
形态学鉴定
UP-PCR
遗传多样性
Fusarium
morphological identification
Universally Primed PCR
genetic diversity.
