摘要
本实验以再生率作为考察指标,采用单因素和响应面法优化了褐黄孢链霉菌原生质体再生培养基中的三种成分,确定其最佳浓度组合为:磷酸二氢钾0.13g/L、L-脯氨酸5.48g/L、聚乙烯吡咯烷酮0.18g/L。在优化后的再生培养基中原生质体再生率达到17.2%,与模型预测值16.7%拟合较好。结果表明,响应面法是优化原生质体再生培养基的一种有效方法。
In order to promote the regeneration rate, three components of protoplast regeneration medium for Streptomyces gilvosporeus were optimized by means of single-factor design and response surface methodology. The results showed that the optimal concentrations of KH2PO4, L-proline and polyvinylpyrrolidone are 0.13, 5.48 and 0.18 g/L, respectively. Under the above conditions, the protoplast regeneration rate reaches 17.2%, which well accords with the model prediction value of 16.7%. It was indicated that response surface methodology is an effective way to optimize regeneration medium composition of protoplast.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第7期154-158,共5页
Food Science
基金
天津市应用基础研究计划面上项目(07JCYBJC07800)
IFS国际科学基金项目(F/4239-1)
天津科技大学引进人才科研启动基金项目(20060426)
关键词
褐黄孢链霉菌
原生质体
再生培养基
再生率
响应面法
Streptomyces gilvosporeus
protoplast
regeneration medium
regeneration rate
response surface methodology
