摘要
探讨血及肝组织中TNF一β的基因表达对大鼠肝移植后急性排斥反应的诊断价值。方法：采用袖套式技术建立不重建肝动脉的大鼠原位肝移植模型，应用RT—PCR方法检测血及肝组织中TNF一β的基因表达。结果：排斥组血中有TNF一β基因表达对大鼠肝移植急性排斥反应有较高的诊断价值，而肝组织中TNF一β基因表达对肝移植后急性排斥反应的诊断无帮助。
Objective:This study was to determine the value of detecting of the gene expression of TNF-β in blood and intragraft for diagnosis of acute rejection after liver transplantation. Methods:We established a rat liver transplantation model without revascularization of the hepatic artery using the cuff technique. Then we used the RT- PCR technique to detect the gene expression of TNF- β in blood and intragrart. Results:The rejective group had the gene expression of TNF-β but the nonrejective group had not in blood(P〈O. 001). Conclusion:The TNF- β gene expression in blood is applicable for diagnosis of acute rejection in rat liver transplantation,but the gene expression of TNF-β in intragraft was helpless to diagnsis ofacute rejectlon.
出处
《肝胆外科杂志》
1998年第2期120-122,共3页
Journal of Hepatobiliary Surgery
基金
美国中华医学基金
关键词
肝移植
急性排斥反应
诊断
TNF-Β
基因表达
Liver Transplantation Rat Acute Rejection Diagnosis Tumor Necrosis Factor-β Gene Expression
