摘要
目的讨论干扰素的抗胶原蛋白合成作用的机制．方法以含小鼠胶原α２（Ⅰ）基因上游０４ｋｂ，０８ｋｂ，２０ｋｂ启动片段为研究靶序列，应用ＣＡＴ作报告基因，结合基因转染技术讨论ＩＦＮα，ＩＦＮγ对胶原基因的启动抑制活性．结果ＩＦＮα，ＩＦＮγ单独应用对小鼠胶原α２（Ⅰ）基因上游不同长短启动序列转录活性无阻断作用，但ＩＦＮα，ＩＦＮγ与ＴＮＦα联合应用则具有较强的抑制活性．结论ＩＦＮα，ＩＦＮγ的抗纤维化活性与其对小鼠α２（Ⅰ）胶原－２ｋｂ～＋５４ｂｐ启动活性的作用无直接相关，但ＩＦＮα，ＩＦＮγ可协助ＴＮＦα的抑制活性．
AIM To study the effect of interferon on the promoter activity in mouse α2(Ⅰ) procollagen gene. METHODS The upper stream 400bp, 800bp, 2000bp sequences in mouse α2(Ⅰ) procollagen gene 5'flanking region were studied with CAT as reporter gene and gene transfection method. RESULTS IFNα and IFNγ had no inhibitory effect in various length promoter sequences of mouse α2(Ⅰ) procollagen gene, while the combination of IFNα,IFNγ and TNFα exerted obvious inhibitory effect. CONCLUSION The anti fibrotic effect of IFN might have no direct relationship with its action on promoter sequence of mouse α2(Ⅰ) procollagen gene. But the interferons have inhibitory effect on the promoters only when they are used in combination with TNFα.
基金
国家自然科学基金
关键词
干扰素
药理学
纤维变性
3T3细胞
interferon alpha/pharmacology
interferon type Ⅱ/pharmacology
promoter regions(genetics)/drug effects
procollagen/genetics
fibrosis/drug therapy
3T3 cells
