摘要
目的:探讨PPARγ激动剂吡咯列酮对重症急性胰腺炎大鼠肺组织ICAM-1表达的影响及其意义.方法:将54只健康雄性SD大鼠随机分为假手术组(A组)、SAP组(B组)、吡格列酮治疗组(C组),n=18,制作SAP模型,通过免疫组织化学方法动态观察3组大鼠肺组织中ICAM-1的表达,同时观察肺组织病理及髓过氧化物酶(MPO)的改变.结果:A组大鼠肺组织出现明显的充血水肿和中性粒细胞增多,而T组肺部炎症反应明显减轻.T组肺组织MPO明显低于A组(6h:5.12±0.71vs6.03±0.63,P<0.05;12h:5.20±0.66vs8.05±0.62,P<0.01).从造模3h起,A组大鼠肺部ICAM-1即持续上调,呈时间依赖性,3,6,12h表达分别为0.82±0.23,1.03±0.31,1.40±0.58,12h与3h比较差异有统计学意义(P<0.05);T组ICAM-1在3,6,12h表达分别为0.62±0.18,0.75±0.27,0.78±0.31,12h明显低于A组(P<0.05);C组呈弱阳性表达,并随时间推移而消失.结论:SAP合并肺损伤时,肺组织中ICAM-1过度表达,中性粒细胞大量浸润,吡格列酮对其的表达有抑制作用.
AIM： To investigate the expression changes of pulmonary intercellular adhesion molecule-1 （ICAM-1） after pioglitazone treatment in rats with severe acute pancreatitis （SAP）.METHODS： A total of 54 Sprague Dawley rats were randomly and divided into 3 groups, named group A, C and T （n = 18）. Group A and C served as SAP model group and sham operation group, respectively. Rats in group T were treated with pioglitazone, an agonist of peroxisome proliferator activated receptor. The modified Li Qing-hua＇s method was used to re- produce SAP models. Histopathological changes of pulmonary tissues were examined by microscopy. The activity of myeloperoxidase （MPO） in pulmonary tissues was measured. The expression of pulmonary ICAM-1 was determined by immunohistochemical staining （ABC）.RESULTS： The histological examination revealed intensive inflammatory response in pul- monary tissues after SAP model was induced, but inflammatory response was significantly alleviated in group T. The activity of MPO （6 h： 5.12 ± 0.71 vs 6.03 ± 0.63, P 〈 0.05; 12 h： 5.20 ± 0.66 vs 8.05 ± 0.62, P 〈 0.01） in the lung tissues were significantly decreased. After modeling, the expression of ICAM-1 was persistently increased from the 3rd hour on. In group A, the expression of ICAM-1 was 0.82 ± 0.23, 1.03 ± 0.31 and 1.40 ± 0.58 at the 3rd, 6th, and 12th hour, respectively, and there was significant difference between those at the 12th and 3rd hour （P 〈 0.05）. In group T, ICAM-1 expression was 0.62 ± 0.18, 0.75 ± 0.27 and 0.78 ± 0.31 at the 3rd, 6th, and 12th hour, respectively, and there was marked difference at the 12th hour between group A and group T （P 〈 0.05）. ICAM-1 expression was weakly positive in group C, and faded away as time went by.CONCLUSION： The severity of pancreatitis and degree of lung injury could be predicted by deictection of ICAM-1 expression and pioglitazone inhibits the expression of ICAM-1 in SAP.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第7期667-671,共5页
World Chinese Journal of Digestology
关键词
重症急性胰腺炎
肺损伤
吡格列酮
细胞
间黏附分子-l
Severe acute pancreatitis
Lung injury
Pioglitazone
Intercellular adhesion molecule-1
