摘要
用不同的试验终点对灵芝的抗突变作用进行了检测，结果表明，灵芝水提取液（ＡＥＧＬ）对多种诱变剂所致的突变具有显著的抑制作用。ＡＥＧＬ对环磷酰胺（ＣＰ）诱导的小鼠骨髓嗜多染红细胞（ＰＥＣ）微核效应有显著的抑制作用，并呈剂量反应关系。ＡＥＧＬ剂量为１０ｇ／ｋｇ时对ＣＰ诱导的微核效应抑制率达６６．１２％。ＡＥＧＬ剂量在６．２５～１００ｍｇ／皿范围内，对诱变剂４—硝基喹啉—Ｎ—氧化物（ＮＱＮＯ）和２—氨基芴（２—ＡＦ）诱导的鼠伤寒沙门氏菌组氨酸缺陷型回复突变有显著的抑制作用，并呈剂量反应关系。高剂量组（１００ｍｇ／皿）的回变菌落数接近于自然回变数。１ｍｇ／ｍＬ的ＡＥＧＬ对丝裂霉素Ｃ（ＭＭＣ）诱导的人外周血淋巴细胞（ＨＰＢＬ）姊妹染色单体交换和染色体畸变的抑制率分别为７０．７％和７６．５％。
Abstract The aqueous extract
from the cultured fruiting bodies of Ganodema
lucidum(AEGL)showedrelatively high antimutagenic activities in of
genotoxicologic tests.AEGL in the dose range of0.4～50g/kg
significantly inhibited the micronuclear ftequency(MNF)in the bone
marrowpolychromotic erthrocyte(PEC) of NIH mice induced by
cyclophosphamide(C.The dose-response relationship was observed and
the inhibitory ratios were 23.68%～6.12%,in theabove dose range.
Strains TA97, TA98 and TA100 were usde in Ames test. The
revertantsinduced by 4-nitro-quinine-N-oxide(NQNO)(-S-9)and
2-aminofluorene (2-Af)(+S-9)on these strains, were significantly
reduced by the addition of AEGL on the plates.Furthermore, the
relation of dose-dependece was observed.The lowest effestive dose
was6.25mg/plate(as crude drug)AEGL also has inhibitory effects on
sister chromatid exchange(SCE)and chromosome aberrotion(CA)in human
peripheral blood lymphocyte(HPBLC)inducedby mitomycin,which of the
optimum concentration of AEGL is lmg/ml(final concentration).The
inhibitory ratios of SEC and CA were 70.70% and 76. 50% respectively.
出处
《中国食品卫生杂志》
1994年第2期1-4,63,共5页
Chinese Journal of Food Hygiene
