摘要
Objective To observe the restoration of structure and function of auto transplanted lymph nodes. Methods Inguinal lymph nodes in Spregue Dawley (SD) rats were auto transplanted by free implantation, or with an intact vascular pedicle, or by free transplantation with microvascular anastomosis, to the popliteal fossa where lymph nodes were removed. The observation methods included emission computerized tomographic (ECT) scanning, staining of China ink and methylthionine chloride to observe the histological changes. Results After four weeks, these vascularized nodes showed normal histological appearances and spontaneously reestablished afferent and efferent lymphatic reconnection with the surrounding lymphatic vessels. ECT lymphoscintigraphy with 99 mTc Dx showed that vascularized lymph nodes had restored their normal function. Conclusion Vascularized lymph node transplantation is a useful method for draining extremity lymph edema.
Objective To observe the restoration of structure and function of auto transplanted lymph nodes. Methods Inguinal lymph nodes in Spregue Dawley (SD) rats were auto transplanted by free implantation, or with an intact vascular pedicle, or by free transplantation with microvascular anastomosis, to the popliteal fossa where lymph nodes were removed. The observation methods included emission computerized tomographic (ECT) scanning, staining of China ink and methylthionine chloride to observe the histological changes. Results After four weeks, these vascularized nodes showed normal histological appearances and spontaneously reestablished afferent and efferent lymphatic reconnection with the surrounding lymphatic vessels. ECT lymphoscintigraphy with 99 mTc Dx showed that vascularized lymph nodes had restored their normal function. Conclusion Vascularized lymph node transplantation is a useful method for draining extremity lymph edema.
