摘要
为测定谷物中伏马毒素含量，了解粮食中有关真菌的污染状态，评价伏马毒素的ＥＬＩＳＡ测定法。采集市售大麦、玉米及饲料玉米，采用酶联免疫吸附法测定，对阳性样品进行真菌培养分离，并用ＨＰＬＣ进行确证。结果：４０份大米中未检出伏马毒素，７０份玉米检出阳性样品２７份，均数０．６８ｍｇ／ｋｇ。ＨＰＬＣ法的确证结果表明２７份ＥＬＩＳＡ法阳性样品中２３份检出伏马毒素Ｂ１，均数为０．４２ｍｇ／ｋｇ；６份同时检出伏马毒素Ｂ２，均数为０．２ｍｇ／ｋｇ。２７份阳性样品真菌分离培养结果均检出串珠镰刀菌。结论：玉米受串珠镰刀菌污染较重，并可有伏马毒素产生。本次调查大米中未检出伏马毒素，情况较好。
おo determinate fumonisin B_1 in cereals, to learn the extent of fungal contamination in cereals, and to evaluate the method of ELISA in the determination of fumonisions. Method:The Samples were collected from market and determined by ELISA kit. The positive ones were confirmed by mycological separation and culture, and determined by HPLC. Result: No fumonisins was detected in 40 rice samples, but 27 out of 70 corn samples were positive with all average concentration of 068mg/kg. The positive samples were also determined by HPLC, and 23 were formed to contain fumonisin B1 (average 042mg/kg) in which 6 sample were found fumonisin B2 (average 02mg/kg). In all the positive samples, F. moniliforme was identified by culture. Conclusion: Corn was easily contaminated by F.moniliforme which produced fumonisins. Although all rice samples were negative, further investigation is need to determine fumonisins in other cereals.
出处
《卫生研究》
CAS
CSCD
北大核心
1998年第S1期116-116,共1页
Journal of Hygiene Research
