摘要
实验选用大鼠骨骼肌缺血再灌注模型， 观察骨骼肌缺血及再灌注后酶组织化学和超微结构的变化， 并观察维拉帕米的保护作用。结果显示： 骨骼肌缺血６ｈ 时， 骨骼肌细胞ＳＤＨ、ＣＣＯ、Ｃａ２＋ －ＡＴＰａｓｅ活性呈下降趋势， 而ＬＤＨ 活性则有所增强。再灌注１２ｈ 时， 骨骼肌细胞ＳＤＨ、ＣＣＯ、Ｃａ２＋ －ＡＴＰａｓｅ 活性进一步明显下降，同时ＬＤＨ 活性亦下降明显，而应用维拉帕米能在一定程度上保护上述酶的活性。与此同时，骨骼肌超微结构的改变与其酶活性的变化相一致。因此， 本实验提示： 骨骼肌缺血再灌注可损害其能量代谢酶的活性， 而维拉帕米则有较强的保护作用。
In this experiment, the model of skeletal muscle after ischemia and reperfusion was used to observe the enzymehistochemical changes in skeletal myocytes and the effects of verapamil on them. The ultrastructual changes were also observed. The results showed that after 6 hours of ischemia, the activities of SDH、COO、Ca 2+ ATPase were decreased, while the activity of LDH was enhanced. Following 12 hours of reperfusion, the activities of all of those enzymes including LDH were further decreased evidently. During the period of ischemia and reperfusion, verapamil could inhibit the declining tendency of these enzymatic activities in a great degree. We also found the ultrastructural changes of skeletal muscle were consistent with the enzyme histochemical changes accordingly. These results suggest that the injury of ischemia and reperfusion may damage the activities of enzymes associated with energic metabolism in skeletal myocytes and verapamil has a significant effect to protect them from the injury.\;
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1999年第4期370-375,共6页
Chinese Journal of Histochemistry and Cytochemistry
关键词
骨骼肌
缺血再灌注
酶组织化学
维拉帕米
大鼠
Skeletal muscle
Ischemia and reperfusion
Enzyme histochemistry
Verapamil
Rat
