摘要
本实验以酶组织化学方法（ＳＤＨ、ＬＤＨ、ＡＣＰ、ＡＬＰ）探讨硒对氟引起肾脏损害的拮抗作用。大鼠分为六组，Ａ组常规饮水；Ｂ组饮水含亚硒酸钠２ｍｇ／Ｌ；Ｃ组饮水中含氟化钠１５０ｍｇ／Ｌ；Ｄ、Ｅ、Ｆ组饮水中分别含氟化钠１５０ｍｇ／Ｌ，依次含亚硒酸钠０．５ｍｇ／Ｌ、２ｍｇ／Ｌ、４ｍｇ／Ｌ。８周后断头处死，观察肾脏组织中ＳＤＨ、ＬＤＨ、ＡＣＰ、ＡＬＰ的活性。结果表明，与Ａ组相比，Ｃ组近曲小管ＳＤＨ、ＡＬＰ活性减弱，ＬＤＨ、ＡＣＰ活性明显增加；Ｂ组ＳＤＨ、ＡＣＰ活性正常，基底膜清晰；Ｄ、Ｅ、Ｆ组均能提高ＳＤＨ活性，其中Ｅ组较稳定；Ｆ组ＡＣＰ活性较高。结果说明了氟引起肾近曲小管溶酶体的破坏，而硒（２ｍｇ／Ｌ）能稳定溶酶体膜。
To
study the antagonism of selenium on kidney damage induced by Fluoride, rats were divided
into six groups. Group A drank water as usual; Group B was given water containing Na 2SeO 3
2mg/L; Group C took water including NaF 150 mg/L; Group D, E, F drank water that contained
NaF 150 mg/L and Na 2SeO 3 0 5 mg/L, 2 mg/L, 4 mg/L respectively. 8 weeks later, the rats
were decapitated and the activities of SDH, LDH, ACP, ALP in kidney were measured by
histochemical methods. The results were as follows: Compared with group A, the activities of
SDH, ALP in proximal convoluted tubules were reduced, and the activities of LDH, ACP were
obviously increased in group C; the activity of SDH was increased in group D, E, F, it is statle in
group E and the activity of ACP was at high level in group F. It was suggested that Fluotide
could induce the kidney damage by destroying the membrane of lysosom, and Se (2 mg/L)
could protect the normal functions of renal tubules by stabilizing lysosom membrane.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1999年第1期57-60,129,共5页
Chinese Journal of Histochemistry and Cytochemistry
关键词
硒
氟
肾脏
酶组织化学
大鼠
Selenium
\ Fluoride
\ Kidney
\ Enzyme Rat
