摘要
通过Linker连接呼吸道合胞病毒(RSV)G蛋白的部分抗原决定簇基因与大肠杆菌热不稳定肠毒素B亚单位基因(LTB)融合,构成融合基因,构建原核表达重组质粒并进行表达,并初步研究其免疫反应.构建得到的重组表达质粒,转化大肠杆菌后融合蛋白在大肠杆菌中得到了表达,用SDS-PAGE和Western-blotting对表达产物进行鉴定,融合蛋白的分子量约为25 ku.该蛋白能够被LT特异性抗体识别.成功表达呼吸道合胞病毒部分G蛋白与LTB的融合蛋白,并且该蛋白有一定的免疫原性.
To investigate the immunogenicity of recombinant mucous membrance vaccine of respiratory syncytial virus, a fusion protein gene was constructed by linking G: 130 ～ 230 and LTB gene with a linker and expressed in E. coli. The recombinant plasmid was transferred into E. coli and target protein was expressed in E. coli. The fusion protein was determined by SDS - PAGE and western - blotting. The molecular mass of fusion protein was estimate to be 25 ku. The fusion protein can be specificly recognized by LT antibody.The fusion protein showed certain immunity.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第S3期355-359,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金资助项目(30570929)
昆明理工大学科学研究基金资助项目(2005-8)
关键词
呼吸道合胞病毒
融合蛋白
免疫原性
粘膜疫苗
respiratory syncytial virus (RSV)
fusion protein LTB- G
immunogenicity
mucous membrane vaccine
