摘要
目的通过研究血管内皮生长因子(VEGF)、COX-2和膜相关前列腺素E合成酶(mPGES)在结肠癌中的表达,探讨VEGF、COX-2和mPGES与结肠癌的关系。方法采用实时荧光定量RT-PCR法,对32例结肠癌组织及其相应癌旁组织中VEGF、COX-2和mPGES mRNA的表达进行了研究。结果32例癌组织中,VEGF121表达无变化。VEGF165高表达19例(59.3%);COX-2高表达20例(62.5%);mPGES高表达24例(75%)。32例癌旁组织中,VEGF高表达6例(18.7%);COX-2高表达5例(15.6%);mPGES高表达9例(28.12%)。结论VEGFl65、COX-2和mPGES与结肠癌的发生相关。COX-2和mPGES在结肠癌组织中上调程度存在差异,提示COX-2和mPGES这两种诱导型酶在调节机制上可能存在差异。
Objective To study the expression of VEGF,Cox2 and mPGES in colon cancer.Methods VEGF,Cox2 and mPGES mRNA expression in 32 paired samples(tumor and adjacent normal tissue)were de- termined by using real time RT-PCR.Results VEGF was overexpressed in 19 of 32(59.3 %)tumor tissues compared with that in 6 of 32(18.7 %)adjacent normal tissue;COX2 was overexpressed in 20 of 32(62.5 %) tumor tissues compared with that in 5 of 32(15.6 %)adjacent normal tissue;mPGES was overexpressed in 24 of 32(75 %)tumor tissues compared with that in 9 of 32(28.12 %)adjacent normal tissue.Conclusion Our result suggested that VEGF165,mPGES and COX2 overexpressed in colon cancer.
出处
《肿瘤研究与临床》
CAS
2007年第4期228-230,共3页
Cancer Research and Clinic
