摘要
背景:研究发现镁离子可以促进成骨细胞的生长,但镁合金在体内的相容性和降解性尚不清楚。目的:观察镁锌合金在动物体内的生物相容性及其在体内降解和新骨形成情况。设计、时间及地点:随机对照动物实验,于2008-03/11在上海交通大学附属第六人民医院动物实验室完成。材料:镁锌合金棒由上海奥芮济材料和医学技术有限公司提供,含镁94%,含锌6%。聚丙交酯棒由京同公司提供。方法:48只新西兰大白兔随机分成镁锌合金组和聚丙交酯组,每组24只。在左侧股骨髁钻成直径0.45cm,长1cm的孔,分别植入镁锌合金棒和聚丙交酯棒。主要观察指标:植入前第1天,植入后第1天、第1,2,5,10周测镁锌合金组动脉血镁离子,镁锌合金组和聚丙交酯组白蛋白、碱性磷酸酶、谷丙转氨酶、尿酸、肌酐水平。植入后3,6,12,18周植入材料部位拍摄X射线片,摄片后取股骨髁,脱钙后苏木精-伊红染色组织学观察和骨量分析。结果:镁锌合金组各血清学指标在第1周内均有不同程度提高,但第2周开始下降,变化无显著性意义。两组其他各生化指标变化差异无显著性意义。X射线片在3周显示镁锌合金组材料旁有气体产生,6周发现有成骨。12周时骨密度明显增加,12周气体自行消失。而聚丙交酯组无气体产生,有成骨。18周时镁锌合金组成骨多于聚丙交酯组(P<0.05)。结论:镁锌合金在动物体内降解时有新骨替代形成,具有良好的生物相容性。
BACKGROUND： Magnesium ion can accelerate growth of osteoblasts, but compatibility and in vivo degradation of Mg alloy remain still unknown. OBJECTIVE： To investigate the biocompatibility and in vivo degradation of Mg-Zn alloys as well as bone formation. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was performed at the Animal Experimental Laboratory of the Sixth People＇s Hospital of Shanghai Jiao Tong University between March and November 2008. MATERIALS： Mg-Zn alloys were provided by Shanghai Aoruiji Materials and Medical Technology Co., Ltd., containing 94% Mg and 6% Zn. Poly-L-lactide rods were provided by Jingtong Company. METHODS： A total of 48 New Zealand rabbits were randomly divided into Mg-Zn alloys group and poly-L-lactide rods group, with 24 rabbits for each group. The left femoral condyles were drilled （ φ 0.45 cm × 1 cm） and separately implanted with Mg-Zn alloys and poly-L-lactide rods. MAIN OUTCOME MEASURES： The serum magnesium Mg2＋ was measured in the Mg-Zn alloys group at 1 day pre-operatively, 1 day, 1, 2, 5, and 10 weeks post-operatively. During the same period, albumin, alkaline phosphatase, alanine transaminase, urea nitrogen, and urine creatinine were recorded in the two groups. Thereafter, the samples were photographed using X-ray at 3, 6, 12, and 18 weeks post-implantations, and then condyles of femur was obtained, decalcified, and stained using hematoxylin-eosin staining for analysis of bone mass. RESULTS： Serological indicators in the Mg-Zn alloys group were increased in the first week, but they were decreased from the second week, and there was no significant difference. In addition, there were also no significant differences in biochemical indicators between the two groups. X-ray examination showed that gas was produced surrounding Mg-Zn alloys after 3 weeks. New bones were found after 6 weeks, bone density was increased after 12 weeks, and gas was absorbed after 12 weeks. Otherwise, gas was not detected, but new bones were found in the poly-L-lactide rods group. New bones in the Mg-Zn alloys were more than poly-L-lactide rods group at 18 weeks （P 〈 0.05）. CONCLUSION： New bones are formed when the Mg-Zn alloys are degraded in vivo, and the Mg-Zn alloys have good biocompatibility.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第12期2232-2236,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(30772182)
上海市卫生局青年基金(2007Y24)~~
