摘要
本实验用接种单纯疱疹病毒方法,诱发豚鼠皮肤及唇粘膜疱疹,动态观察接种区疱疹发生及郎格罕细胞 (LC) 的变化。结果表明接种病毒后2～3天,皮肤或唇粘膜即发生疱疹,前音散在出现,持续3～5天;后者成群发生,持续5一8天。皮肤病灶内 LC 数目明显减少,而唇粘膜接种部位内树枝状、ATPase(+)LC 完全消失。病灶周围皮肤或粘膜 LC 随之向病灶迁移,聚集在形成疱疹的部位。至疱疹消失,LC 数目复原。由此提示,在病毒感染早期,LC 可能具有类似巨噬细胞的功能。
To investigate the role of Langerhans cells (LC) in immune response against herpes simlex virus (HSV-1) infection,cutaneous and labial vesicles were induced by inoculating HSV-1 into guinea pigs.The behavior of LC duing the development of lesion were noted continuously The results showed reduction in number or total disappearance of LC at areas of inoculation 1-2 days after infection of HSV-1,followed by migration of LC from adja- cent regions to the sides of inoculation and finally,accumulation at the sites of vesicles The numbers of LC returned to normal when the lesions resolved.This suggested that LC could possibly replace the function of macrophages at early stage of HSV-1 infection.
关键词
单纯疱疹病毒
郎格罕细胞
抗病毒
Langerhans cells
cutaneous and mucosal vesicles
herpes simplex virus
immune response
ATPase staining
