摘要
猪十二指肠类肝素（冠心舒）是一种富含硫酸基的硫酸多糖，对培养的兔主动脉平滑肌细胞增殖以结晶紫、ＭＴＴ染色和３Ｈ－ΤｄＲ掺入等方法测定显示明显抑制作用，作用强度与肝素相仿。同浓度的右旋糖酐对此无抑制作用，说明猪十二指肠类肝素抑制血管平滑肌增生与分子中所含硫酸基有关。猪十二指肠类肝素口服有效，抗凝效价又低于肝素，提示本品有可能用于防治动脉粥样硬化和血管成形术后再狭窄。
The authors investigated whether heparinoid abstracted from porcine duodenum inhibits the proliferation of rabbit aortic smooth muscle cells (SMCs) in vitro.Measurement of growth of SMCs was monitored by crystal violet staining,MTT method and 3HTdR incorporation.Proliferation of SMCs was induced by 10% fetal calf serum(FCS).The results showed that heparinoid at 0.2￣0.05mg/ml significantly inhibits the proliferation of SMCs.Pretreatment with heparinoid for 24h,the SMCs proliferation induced by FCS was also inhibited.The results suggest that heparinoid may be useful in the prevention and treatment of atherosclerosis and angioplasty restenosis.
出处
《山东医科大学学报》
1998年第1期80-84,共5页
Acta Academiae Medicinae Shandong
基金
国家自然科学基金
